FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_87.35100000042a18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_87.35100000042a18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119442
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC43343.6285393747592973No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG22011.8427353862125553No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT14141.1838381808743992No Hit
GTATAAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT6040.5056847675022186No Hit
GTAGTATAGTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTT5000.4186132181309757No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC3790.3173088193432796No Hit
GTTATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTAT3210.2687496860400864No Hit
GTATATATACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT3090.258702968804943No Hit
CTTTAATATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT2330.19507375964903467No Hit
GTATAGTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT2170.18167813666884344No Hit
CTTTCTATACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT2090.17498032517874784No Hit
CTTCTAATACGTTTTGGTAAAGAAGAGAGTATAAAGACTGTCTCTTATACA1790.14986353209088932No Hit
TAATAGTACACAGTATTAGCTGTCTCTTATACACATCTCCGAGCCCACGAG1700.14232849416453175No Hit
GAATTAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT1420.1188861539491971No Hit
TTGTAGTATAGTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTC1330.11135111602283954No Hit
CTATATTACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTA1320.1105138895865776No Hit
ATATATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTA1250.10465330453274392No Hit
GCATAAATACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT1240.10381607809648197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4650.043.5479845
TAATACG707.9853635E-1032.142565
ATACGTT707.9853635E-1032.142567
ATGCCGT6450.031.39505844
TGCCAAT300.005129252629.9997215
CGTTTTG751.6716513E-929.9997210
GGTAGTA300.005129252629.9997212
TGGTAGT300.005129252629.9997211
GTTATTT803.3160177E-928.136521
GTACAGA507.0859154E-527.0110591
GGTAAAG856.359187E-926.47034316
TTGGTAA856.359187E-926.47034314
AATACGT856.359187E-926.4703436
TATATAT608.112082E-626.2497562
TTCTAAT901.1685188E-824.999772
TAGTACA450.001220192624.999774
CTTCTAA1101.4733814E-1024.555511
TATGCCG8600.023.80791943
TCTAATA952.0756488E-823.683993
TGGTAAA952.0756488E-823.6839915
Produced by FastQC (version 0.11.2)