FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_86.351000000429e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_86.351000000429e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331526
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC81292.4519947153466095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG9440.28474388132454165No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT9330.28142589118198874No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8630.26031140845665196No Hit
GATATACACATTCTGATGTTGTTTATAAAAGGCCAATTAGGGATTTAGATA8260.24915089615897396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.18550581251545883No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5970.18007637410037222No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5610.16921749727019902No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4630.13965722145472753No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.13633923131217462No Hit
ATTAGATACAACGCTAAGAGAAGACACTGGAAGAGAACTAAGTTAGGTATC3780.11401820671681859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.10647732002919831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.10104788161411171No Hit
GATTTAAGACTGGTAACACAATTAGATACAACGCTAAGAGAAGACACTGGA3320.10014297521159729No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8100.041.3855645
ATGCCGT9200.036.19273844
TCACGCA250.002099552436.0188374
GTCTATA250.00210578235.99710537
GTGACGT250.00210578235.99710530
GATATAC1300.034.6387251
CTCGTAT10100.033.4131539
GTATGCC10150.033.24855442
TATGCCG10100.032.96764443
ATCTCGT10350.032.8234537
TATCTCG10500.032.35453836
TCTCGTA10600.032.26155538
CGTATGC10600.031.8370641
TCGTATG10600.031.8370640
GTATCTC10650.031.47634135
TAGTATC10800.031.03917133
GACGTCT10900.030.54826
ACGTCTT10850.030.48141727
CTTAGTA10950.030.40851231
GTCTTAG10900.030.34159529
Produced by FastQC (version 0.11.2)