FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_85.351000000429c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_85.351000000429c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247726
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC148786.005829020772951No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG57932.3384707297578777No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT32211.3002268635508585No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG9240.37299274198105975No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA8790.3548275110404237No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG7450.3007354900171964No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA6660.26884541792141314No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5050.20385425833380427No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC4870.19658816595754988No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTA4380.17680824782219065No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3880.1566246578881506No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTA3850.1554136424921082No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3520.1420924731356418No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC3500.14128512953828018No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3500.14128512953828018No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA3480.14047778594091861No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG3410.137652083350153No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG3360.135633724356749No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA3360.135633724356749No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC3200.12917497557785618No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA3040.12271622679896337No Hit
ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3040.12271622679896337No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC2990.12069786780555937No Hit
GTGCATTTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT2960.11948685240951695No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA2940.11867950881215537No Hit
GCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCG2790.11262443183194334No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT2720.10979872924117774No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT2700.10899138564381616No Hit
GCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC2680.10818404204645454No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG2600.10495466765700814No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT2590.10455099585832736No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA2590.10455099585832736No Hit
TTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA2490.10051427787151933No Hit
GTGTTGGGTTGACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG2480.10011060607283853No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACT253.8781138E-545.007474
GACAGCC207.0238026E-444.99838610
TAGTCCT207.0238026E-444.9983867
TGCCGTC16350.043.3470745
CAGGGTG301.1362269E-437.506224
GTGGGGC301.1375786E-437.49865316
CCGTACA301.1375786E-437.49865317
TGGGGCC301.1375786E-437.49865317
CGTACAC301.1375786E-437.49865318
ATGCCGT19100.037.10599544
TCCCTCC250.002104296635.99870724
GGTGGGG250.002104296635.99870715
GGACCTC702.0008883E-1135.35587319
GAGTCTC458.6247746E-735.019951
ACGTTTC653.6379788E-1034.621136
GGGGTGC401.55603E-533.74878718
CGGGATT401.55603E-533.74878717
ACAGGGT352.8112947E-432.1481933
TACGTTT708.058123E-1032.1481935
CGTTTCT708.076313E-1032.14177
Produced by FastQC (version 0.11.2)