FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_84.351000000429ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_84.351000000429ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244390
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC65312.672367936494947No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG17340.7095216661892876No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT8360.3420761896967961No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3630.14853308236834567No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC3000.12275461352755841No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.11170669831007815No Hit
ATCTTACAGCATGTGTGTTGGAAGATGAGAAGTTAAAACTGAGTTATAAGA2600.10638733172388395No Hit
GTATTAATGCTTGTGATATCTAAGTAGTTAGTTGTGATATCTATGCCATGG2570.10515978558860836No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC207.015926E-445.008391
GCGACGG207.023005E-444.9991812
CCTATCA207.023005E-444.9991836
TGCCGTC6800.042.6830545
TCTAGCA356.220145E-638.5786172
GTCCTCA301.1374147E-437.4993242
ATGCCGT7850.036.9738544
GCCCTAT250.00210405835.99934434
CGTCCAC250.00210405835.9993446
TGCACAG250.00210405835.99934422
TGTCCTC352.8142246E-432.1422741
CTAGCAC501.9703166E-631.5058733
TCGGAGC300.005134736730.0055964
TGCCCTA300.00513987929.99945433
CTACTTG300.00513987929.99945427
TTTCGTG300.00513987929.99945413
GGGAGGT453.480154E-529.99945414
ACGGATC300.00513987929.99945415
GTCCACT300.00513987929.9994547
TATGCCG9700.029.92213443