Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_1228_pb_84.351000000429ad.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 244390 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC | 6531 | 2.672367936494947 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG | 1734 | 0.7095216661892876 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT | 836 | 0.3420761896967961 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 363 | 0.14853308236834567 | No Hit |
GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC | 300 | 0.12275461352755841 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 273 | 0.11170669831007815 | No Hit |
ATCTTACAGCATGTGTGTTGGAAGATGAGAAGTTAAAACTGAGTTATAAGA | 260 | 0.10638733172388395 | No Hit |
GTATTAATGCTTGTGATATCTAAGTAGTTAGTTGTGATATCTATGCCATGG | 257 | 0.10515978558860836 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCTAGC | 20 | 7.015926E-4 | 45.00839 | 1 |
GCGACGG | 20 | 7.023005E-4 | 44.99918 | 12 |
CCTATCA | 20 | 7.023005E-4 | 44.99918 | 36 |
TGCCGTC | 680 | 0.0 | 42.68305 | 45 |
TCTAGCA | 35 | 6.220145E-6 | 38.578617 | 2 |
GTCCTCA | 30 | 1.1374147E-4 | 37.49932 | 42 |
ATGCCGT | 785 | 0.0 | 36.97385 | 44 |
GCCCTAT | 25 | 0.002104058 | 35.999344 | 34 |
CGTCCAC | 25 | 0.002104058 | 35.999344 | 6 |
TGCACAG | 25 | 0.002104058 | 35.999344 | 22 |
TGTCCTC | 35 | 2.8142246E-4 | 32.14227 | 41 |
CTAGCAC | 50 | 1.9703166E-6 | 31.505873 | 3 |
TCGGAGC | 30 | 0.0051347367 | 30.005596 | 4 |
TGCCCTA | 30 | 0.005139879 | 29.999454 | 33 |
CTACTTG | 30 | 0.005139879 | 29.999454 | 27 |
TTTCGTG | 30 | 0.005139879 | 29.999454 | 13 |
GGGAGGT | 45 | 3.480154E-5 | 29.999454 | 14 |
ACGGATC | 30 | 0.005139879 | 29.999454 | 15 |
GTCCACT | 30 | 0.005139879 | 29.999454 | 7 |
TATGCCG | 970 | 0.0 | 29.922134 | 43 |