FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_8.3510000004203c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_8.3510000004203c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51448
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC50259.767143523557767No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT8761.7026900948530554No Hit
ATCTTCATCAGAGTCTTGAAGAATTTGTTCTTTTGTTGAAATTCCTGCAAT4920.9563053957393872No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG2190.42567252371326386No Hit
GACACTAATGGACTTAATGAGTTTGTCACATAACAGTCAAATGTCAGTATT1760.34209298709376457No Hit
AAATGGATCTTCAACAAATGTTTCTTCTGTTAACTGGCAGATATTATCTGG1740.3382055667858809No Hit
GTTATACTAACAGCTACAACTACTGGTAATGGAACATGTAGTCCTATTGTT1700.3304307261701135No Hit
TCACTAAGCAATGTGTTAATCCGTTGTTCTATTAATGCTTTTGAAGTACCC1560.3032187840149277No Hit
AAATCATCCTGATAATATAGCACCTGTAAGCAACCAAGATATTTGGTATGC1560.3032187840149277No Hit
TAAGAGATTCTGCAACGATAAGAAAAATGTTCCATATATTGCTGTGGAAAC1550.30127507386098584No Hit
AAATAAGTTGATTTGCAGTTTGAAGGATAAAACAACAAAACTACTGTTTTA1510.29350023324521846No Hit
GTTTTAGATGCTGATACTTATGTTGATTGGACGGATAGTTTATTGAAAAAA1450.28183797232156743No Hit
GTATTTCCATATAATCGCTCAACAACTTAGTATGCTTTTGACGAACATCTT1410.27406313170580004No Hit
TGTTATTTTGATGATGGCCTTTTATTCTTCTCTTTTAACTTCCATTGATGG1410.27406313170580004No Hit
TATGATTTCCTTTTTGTTGACGGAGTTTTCAAATGCTGTGTTCAAATATCA1400.2721194215518582No Hit
AAACTATTTTGGTGATACTCCTTTAAAGCCATTTAAAGATGCGGATGATCT1340.2604571606282071No Hit
GTTTTAACTCATTTTCAATCGTTCTTAGCAATTCAAAGGTTATCATGAAGC1270.2468511895506142No Hit
ATGTACAACAGGTAACAGATTAAAAGGATCAGCACTAAAAAAAACCACATC1270.2468511895506142No Hit
CATAAGCATTGGGAAAATTTAGAAAAACTTCGTAAAGCAGAATGGTTGGAA1260.24490747939667237No Hit
AAATACCCTGATAAGAAAGGTTATTCCACTCGTAACCTTATCTATATGTGC1190.23130150831907945No Hit
TTTGTTTTACCATAGTCCTTGATGGTTTTTTTTAATTGTTGCTGGTAACTT1150.22352666770331206No Hit
CTTTTCTATTGATCAAAGAGGAAAATATGTGAACGCTAAGGAAATGAATCA1130.2196392473954284No Hit
GAGTTAATACACTACGGCTGATTTTTACTACGTGATTAAGCTCTATAAGTA1030.20020214585600998No Hit
GATATCTACATAAACAATATCTCCCAATTCATGCTGTGCAAAATCGGTAAT1000.19437101539418442No Hit
GTTTTATTTCCAAATGGGTTGTTTTAGGAACTATAGTACTTGGACTGCTAG970.18853988493235888No Hit
TTCATGTTTTACAACATGAAGATAGAACCGAAATAGGTAACTACATTTTCT960.18659617477841706No Hit
ATGGATATGTTTGATTAATACAGCTTTGAATGGCTTGTGATAGATAATGCG960.18659617477841706No Hit
ATTTCATATAGTGTCGTTTCGACATTGGGAATATTTACATCTGCTCCGATT870.16910278339294044No Hit
GGAATTACACTGAAGGAACATATTTTTCAGTTATGACAGTCTCAGTCACTG820.15938423262323123No Hit
ATCCATACTAATGCTAGTGTAGATACGATACTTGTGTATAGCAAGAAAATG810.15744052246928936No Hit
AAGATGAATTTAGTGAAGACCTCAAAGATGAATTTGAAAATTCAAACGATG800.15549681231534754No Hit
AATCGGAGTAATCTGAATGATTCCTTTTTCTAAATCGACTACTGGGATAAA800.15549681231534754No Hit
ATGATATTCAGTATGATGCGATAAAGAAGGCTTTGGGAAAATAGTTTCTAG780.15160939200746384No Hit
GACATATACTGCTCCAACAAAAATATGTATCTGAAATAGCCTCACCATTTA780.15160939200746384No Hit
TGTATCAACACCAAGAATAGAGTTGCAAATTAAGTGATCTGAAATACGAGA770.149665681853522No Hit
ATACTTTTAAGGACATAATCATGGTTTTCTACTTTTCTGTGGAGCCTGAAA760.14772197169958015No Hit
ACATTATTTCTGCAGTGGGGTTTTTAAAATCTTACTACAGTCACAGGGAAA750.1457782615456383No Hit
TATTAGAAGAAAGATCCATGAAAATCCAGAATTAGCTGGCAAAGAAGATGA720.1399471310838128No Hit
ATGATATTTTCCGTTCTCAGGTATTTGAGCAATATTCTGAAAGTAGCTTCT720.1399471310838128No Hit
ATTTAGAAAAGAAAATGCAGAACTTGGAAAAGCAAACAACTCTTTCATTAA700.1360597107759291No Hit
GTAGAAGAACTCGATAAAAAAACATTGGTTATTCGAGATGTACCTTATAGT700.1360597107759291No Hit
ATTATGATGAATTTGCAGCTTTTACTTAGTAACTTCATGGAATTCACAAAA680.1321722904680454No Hit
ACATAGTAGAAACTTTACTAAAGGGGGAACAGTTGCTAAAGAAGGACTAGT660.1282848701601617No Hit
CAGATATATCCTCAATTTCTTTACGAACTCTACTCTTAGTCTTCTGCCAAT660.1282848701601617No Hit
GATAGATATGGATAAGAATTGGATTCCTAATATTGCTGATCACTCTTTGTA640.12439744985227802No Hit
ATCTACCATTGTAGTTTCGTTATTGGGTGCATTTGTAGAATGGTTAAATCT640.12439744985227802No Hit
AGATACAAATAACAATGAAGCAGCTTACAATATTCTCAGCAATAGTAACTG630.12245373969833619No Hit
ATCTAAGAATTGGTTTGCAGAATATGTGAACTACCACAACTCAATAATCAA620.12051002954439434No Hit
AACTAAGATTGCACAATTGTCATTTGTCTCTTCTGTTGAACCTGTATCAGT600.11662260923651065No Hit
TACATCCTCAGTTTATGAAGATGATGGGATTAAGAGATTTAAGTAAAGACA600.11662260923651065No Hit
TGTTTGTATATCAAGCTCTTTGTAGATTTCACACTAACCGTCTTGGATACT580.11273518892862695No Hit
AGACAAGAAGACAAACTGTTTACAGATATTATTAGGCCTTTTCAAAAATGC570.11079147877468512No Hit
GTGTTTATACTGTTTCTGTAAATACGGGTAGCGATGGAAATACGGTGTTAG570.11079147877468512No Hit
GATATGTATGGATTTACAACGGATTATAGCGATGAAGAAATCGTTGCTATT570.11079147877468512No Hit
GTATACGTTGTATTTACTTTTCCAGAAGGAATACTAAGCGGACTAATTTCA570.11079147877468512No Hit
ATAGAATACTTTCTAACGCAGAAGTAAACCTTTCTTTTGGTGATCAGGTAC560.10884776862074327No Hit
TGACAAAATATTGATGATTGATGCAAGGAATGTTTTCAGAAAGGTTACTAC560.10884776862074327No Hit
GTGAAAAATTTTAACGAGACAGAAGAACATTTGGATTTCTTGATTAATAAT560.10884776862074327No Hit
AATCTAATTCGTACCTGAATGTCAAGAACATTTCATATAAACTTGCACACA560.10884776862074327No Hit
ATCTTGGTTACTTGAGGTATTTTCTTCTTTAATTGGCTTTTACGATCAACA540.10496034831285958No Hit
GTGGTATGCTAATGACTGTGAATTCTGTGGTCTTTGGAATAGTTCCAAAAT540.10496034831285958No Hit
CTTCTTACTAAACTGACTTTGTTTTTTCTTGTTTTTTCTGAGCGGGAAATA530.10301663815891775No Hit
CATAGTATAGCAGTATAAGCATAACGTAGTAACTCCCCAGGGTTTGATATA520.10107292800497589No Hit
GAAAGAAACTTAACCCTTCATTTTCCATAGAAGTAACCATTTGGTCTTGAA520.10107292800497589No Hit
TGTACAAAATCTAGAGCAAAAGTTTGGAAATGAACTAAACATTTAGTCTAG520.10107292800497589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATT302.112496E-645.038952
CATATAC253.8192502E-545.0389483
GAGATTC253.8192502E-545.0389484
TCTGCAA253.841317E-544.9951369
ACAACTC253.841317E-544.99513636
ACAACTA206.97422E-444.99513616
GGAATAT253.841317E-544.99513628
TTAGGAG206.97422E-444.99513631
AGTCCTT206.97422E-444.99513614
AGTCCTA206.97422E-444.99513640
AACCGAA206.97422E-444.99513626
GTTCTCA253.841317E-544.99513613
GAAGAAT502.0008883E-1144.99513618
GCTGGTA206.97422E-444.99513641
ACCATAG206.97422E-444.9951369
TAGGTAA206.97422E-444.99513634
ATGGAAC206.97422E-444.99513629
ATCAGAG502.0008883E-1144.9951367
TAGAACC206.97422E-444.99513623
ACCTGGC206.97422E-444.99513615
Produced by FastQC (version 0.11.2)