FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_79.35100000042919.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_79.35100000042919.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233253
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC50042.1453100281668402No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG25481.092376089482236No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT10940.4690186192674906No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGC5750.24651344248519846No Hit
ATATAAGATCATCTCTTACTTTACAAACAACAATGGCTGTCTCTTATACAC3000.12861570912271225No Hit
GAACATCTTTATTTCTGCCTTCATTTCATTATGTACCCAGTAGTCACTGTC2740.11746901433207718No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC207.008864E-445.016733
AGGGGTA207.023692E-444.9974323
TGCCGTC5050.042.76983345
CGTGAAA250.002104264735.99794431
ATGCCGT6350.034.013844
ACCCTTA300.00513499830.0047176
ACATGGC300.005140385629.99828527
CTCTGTG300.005140385629.99828543
CCTTAAT507.105951E-527.0158311
AGTGACC507.137153E-526.9984578
GTGATGC450.001224032624.9985719
GGTTGTC651.517668E-524.2293837
TATGCCG9150.023.60520743
GTATGCC9300.023.466442
TAACCCT602.453156E-422.5083644
AGACCTT500.002257178522.5035386
TTATCGA602.4602903E-422.49871429
GATGACT500.002259993522.49871434
TATCGAT500.002259993522.49871430
CCGTTGT500.002259993522.49871433