FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_77.351000000428d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_77.351000000428d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301764
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC223687.412414999801169No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT106113.5163240147930175No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG21620.7164539176309964No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA3410.1130022136504023No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGC3250.10770005699818402No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3030.10040959160138385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC20500.044.77949545
CCGTCTT356.2339313E-638.57057627
CACGGTT301.1380758E-437.49917223
GTGGACG250.002104880335.99920725
CGGATTA250.002104880335.99920744
CCCGTCT401.5570053E-533.74925626
GACCGGG352.8158474E-432.14214740
TGACCGG352.8158474E-432.14214739
GGTGGCG352.8158474E-432.14214745
ATGCCGT29950.030.6504144
AGTAGAG300.00513770530.0043073
TTGTCGG300.005141870629.99933840
GTATCAG300.005141870629.99933815
ATGCGTC300.005141870629.9993387
CGATGCG300.005141870629.99933814
TGTCGGA300.005141870629.99933841
CTCAGGT300.005141870629.99933821
ATCTGCC300.005141870629.9993387
GTCGGAT300.005141870629.99933842
CGACGAT453.482933E-529.99933623