FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_76.351000000428ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_76.351000000428ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231473
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC44151.9073498852997977No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT5970.25791344994880616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.1689181891624509No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG3870.16719012584621099No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3410.14731739770945207No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2790.12053241630773351No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.11189209972653398No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.10281976731627447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.1023877514872145No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4450.043.98348645
ATGCCGT5000.039.14529844
TATAGCC301.1321323E-437.5279272
TATGCCG5450.036.32591243
GGCTTAT250.002091518636.0423971
GTATGCC5550.035.671442
CTCGTAT5550.035.2660439
ATCTCGT5750.034.03939437
TATGCTA401.547297E-533.7751355
TCTCGTA5900.033.55529438
CGTATGC5900.033.1739841
AATCTCG5950.032.8952136
CAATCTC6100.032.45511635
TCGTATG6050.032.3514940
CGAGACT6250.031.67619723
GACTGAT6350.031.17735926
ACGAGAC6350.031.17735922
ACAATCT6400.030.93378834
CACGAGA6450.030.69398921
CCACGAG6450.030.69398920