FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_72.35100000042834.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_72.35100000042834.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80828
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC59727.388528727668629No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT28583.535903399811946No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG5540.6854060474093135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA206.994376E-445.039
ATGTATC206.994376E-445.014
ACTAGTG206.994376E-445.07
TTCCATG206.994376E-445.08
CGTACTT206.994376E-445.035
GACATTA206.994376E-445.08
CTCTATG206.994376E-445.09
AGTACGT253.8585888E-545.031
TGCCGTC6500.044.30769345
TGTTTGC250.002095560336.08
CTAGAAT250.002095560336.027
CATTGGA250.002095560336.02
CACTGCA250.002095560336.022
GTTCCTT250.002095560336.011
ACGTACT250.002095560336.034
GTAAGTA250.002095560336.011
GGACATT250.002095560336.07
CAGTAAG250.002095560336.09
AAAGGAC250.002095560336.04
ATGCCAT250.002095560336.06
Produced by FastQC (version 0.11.2)