FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_71.3510000004280d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_71.3510000004280d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180440
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC4459324.71347816448681No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT81734.529483484814897No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG34231.8970294834848147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC47450.044.523945
ATGCCGT55050.038.3770944
CGTATGC59000.035.9221841
ATCTCGT58950.035.91448237
CTCGTAT58900.035.9067739
TATGCCG58900.035.9067743
GTATGCC58900.035.9067742
TCGTATG59100.035.8233340
CTGTATC59150.035.7930533
TATCTCG59100.035.7852636
TCTCGTA59100.035.7852638
AGACTCA59050.035.7774625
GACTCAA59100.035.7471926
ACGAGAC59500.035.73375322
GAGACTC59250.035.73263524
CACGAGA59500.035.6959421
CGAGACT59400.035.6802823
CCACGAG59600.035.67379820
GTATCTC59300.035.66456635
ACTGTAT59500.035.65812732