FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_70.351000000427e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_70.351000000427e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319753
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC201346.29673529255394No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT67852.12195038044991No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG10230.31993444940313304No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5400.16888035452364794No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4520.14135911156423867No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3580.11196142022123327No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3540.11071045463216922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC19700.044.65441545
CTCAGGT250.00210552235.99762321
ATGCCGT27000.032.5811844
TATGCCG27850.031.58678243
ATCTCGT27750.031.45738237
CTCGTAT27900.031.44953539
GTATGCC27950.031.39327642
GTATCTC27900.031.28825635
AACTGTA27850.031.26364331
CGAGACT27900.031.20761723
AGACTCA27900.031.12697625
GAGACTC28050.031.0407324
CACGAGA28200.031.03518321
TCTCGTA28200.031.03518338
ACTCAAC27950.030.99079927
ACGAGAC28250.030.98025722
CCACGAG28250.030.98025720
TCGTATG28600.030.83712840
CGTATGC28400.030.81662641
TATCTCG28300.030.7665236
Produced by FastQC (version 0.11.2)