FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_7.35100000042012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_7.35100000042012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157526
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC95586.0675697980016No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT15650.9934867894823711No Hit
ATACTTAGTGGATAAAACATTAGAGGAAAAACGAAACTTTTGTTCAGTGTT8560.5434023589756611No Hit
CATTTATCATCAATATTTTCCGGATTGATTTCAGAAGGTAGCTTTATTGAC8430.5351497530566383No Hit
GAATAGTATTCCATTGTATGTATGTGTGACATTTTGTTTACCGCACTTTCT8350.5300712263372396No Hit
GTTATTACGTTTCTATTTGCACCTTCTTGAAAATTTCATCATTTAAGTGCA7730.49071264426189964No Hit
ATGTATCAGATTTCGAATAAGGATACCTTGTTTTTGAAAGCAGATACGCTT7240.4596066681055826No Hit
ACTTAGAATGAATTAAGTTTCATTTGGTTTTCTCTACTCTCAACTGTGACC6560.4164391909906936No Hit
GAAAGAAAAGAAAGGAAAGAAAGGAAAGAAAGGAAAGAAAGAAAGAAAAAA6460.4100910325914452No Hit
CTTAAATCCTTTCTTTAAAGCCGTATTAACTAGTCTTGTTGCATCAAAAAA6360.40374287419219684No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6350.403108058352272No Hit
ATTCTATTTGAATAAGCAGGTTTTAAAAACTCTAGCCTAAAACAGGAACCT6220.39485545243324915No Hit
GTTGTAATTCTTGTCCTTTTTTTTACTCTCTGTATTCTTTCAAATGTTCTG5850.3713672663560301No Hit
TCTTTAAGATGACCATTATGGATCCTTTGTCAGTAATCTTATAGATCTCCA5810.3688280029963308No Hit
GGATAAATACTAAAATGTCTACACTTGGGCATATGATAGGAACATTGAAGA5650.35867094955753337No Hit
ATCAAAAAGAATTGAATCAAATGGAATTGAAAAGAATCGAATTGAATCAAA5380.34153092187956274No Hit
GTATATATTTCCTCACCGGCTGTTTAGTATTTCATCTGCATTCCACTTTCT4820.3059812348437718No Hit
ATTACTGGAATAACAACACTAAGATTTTTTCATTTCTGATCTTCATACTTT4810.30534641900384696No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4730.3002678922844483No Hit
AAATTACTTTTAGCTCTTCAAATTTTAGCCATAATCATAATAGTTGCATCT4730.3002678922844483No Hit
CAATAAACAATGAGTAACATTAATATGTTCGATATTTCCAGCAACAAATTA4710.2989982606045986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.29391973388519993No Hit
CAATGTAGCTATAGTGTGATTTACAATAACTTTGTGTTTTTGTTATCAGCA4450.28249304876655285No Hit
GTATTTGTCAATGATAATAAAGTGACCATAGAAAACAGAGACACATTCAAC4410.27995378540685345No Hit
GTATGAATGGTAACTGTAATAAGTTTTAAAGTACGCTCAATAACAATTTTA4300.2729708111676803No Hit
TTGTAATTCTTGTCCTTTTTTTTACTCTCTGTATTCTTTCAAATGTTCTGT4280.27170117948783057No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4180.2653530210885822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.2628137577288829No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3970.2520218884501606No Hit
ACTAATCACCATAGGATCTTGCCATTTGATATTAACGTAGCTATTATTGAC3970.2520218884501606No Hit
CTTCCTAAATAGCAAACATGAAATTGTCCAAACATGATAGTTTAGAGAGCA3920.24884780925053643No Hit
GTCTATGTCCCTAATAAATTCACATTGCCAATGTCAGTGATGATAATTTCA3770.23932557165166385No Hit
TGTGTAAATCATTCTAAGTGATGCAGTAAATTAGTGAACCTACTTCCTAAT3770.23932557165166385No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.23170778157256577No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3620.2298033340527913No Hit
CATGTAAAACTATGTAGTGTTTTACTAGCAAGTACAAAGGGTGTATTGTCT3610.22916851821286643No Hit
CTATTAATATAGGCCATTAGTGCTATAGTATTGTAGATATCACCGTTGATA3460.21964628061399388No Hit
GAAACATGTTGTAATTCTTGTCCTTTTTTTTACTCTCTGTATTCTTTCAAA3330.21139367469497097No Hit
ATTAAAAGGAAAAATCCAATTAACAGAAGAAAACCAAGAAATAGTCACTAC3300.20948922717519647No Hit
ACATTATTGGAGCAAATAATCTTAAAATAGCTTGCCATAGTCCTTTAAATA3270.20758477965542196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG3260.20694996381549713No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3230.2050455162957226No Hit
ATATTATGGGTTCATACCAAAATAGAACATAGAGCAGTTTCTCAATTTTTC3170.2012366212561736No Hit
GTAATAATCTCCACCTGTTTCATAGCTCAAGAAATAGCATCTTTATTTAGC3120.1980625420565494No Hit
CTTATTGAATACTGCACTGAAAATGAAAAATAGAATGTTTGTATGGATACT3000.19044475197745134No Hit
CTTATTAAGCCCTTTATTAAACCGGAAAGTGATTTTTACGGATCGGGATAT3000.19044475197745134No Hit
GTATTGTATTTGCCAACAAATTTAATTTTCTGTTTATGGTTTTCAGGAAAA2960.18790548861775197No Hit
CAACACAAAGGATTATTAAAGGCAATTAAGGTTGAAAAATTTGTCGTAAAC2930.18600104109797747No Hit
ATGCATTTCAACTAATGTATATGGAACCATTTAACAAATATCTCAAGGTAC2920.18536622525805263No Hit
GGTAAAAGCAATTTGAAATTCATGATTTGAGATTTGAGATTGTAACGATTT2830.17965288269872912No Hit
ACTTAATAGTCAAAGACACGATAGTTCTATATTGTATACATGCAATTTTGC2810.17838325101887942No Hit
CATTATTATAATTCAAGAGCATAAGCTTCAGCTCTTCTTTCAAAATTTGTA2770.17584398765918008No Hit
TTTTTACTCTCTGTATTCTTTCAAATGTTCTGTTTGTCTTTGGTTTTCTTC2690.17076546093978137No Hit
ATTTAAAATGAAGCTAAAATTCAGCCCTATAGGAACAAAGAGACATAGAGA2690.17076546093978137No Hit
AGGTTATACAAAGTGAGTTCTGAATCCCATTATCTTCCTTCTTTACCTTAT2690.17076546093978137No Hit
ACTATATAAAGGCAGCATCTTTTAAAAAGTGAAAACATCACCAAAAGATTT2670.16949582925993167No Hit
GACAGAAACATAGGAATCTGTGTTATTCTGTAGGATAAACTCAGTTTTTTT2670.16949582925993167No Hit
GTATATGAAAAAAAAGCTCGACATACTAATCATCATGGAAATGCAAATTAG2670.16949582925993167No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2660.16886101342000684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.16695656590023233No Hit
ATATCAGGATATTCAGAAGCCCATAATAATAAAGAGTTCTCAGTGTACTAG2610.16568693422038266No Hit
GCTCTATATTTTGATATAGATCGACAAGAAATTAGTTTTCATTCATGTCTT2610.16568693422038266No Hit
TCTTAGAGAAAAGTAAGATGTGAAGGGAATGATAAAACCACTAAAAAAGTG2520.15997359166105912No Hit
GAATTAAGGAATGAATGAGCAAACAAATAAACCAATGGGTTTAGTTGATTC2440.15489506494166042No Hit
CAGTAGTTGAAAGTATTTATCGTCAGCACATTCACTGTACAAGAGATATTA2420.15362543326181075No Hit
ATATTGTATACATGCAATTTTGCCCTGCAGCTAAAAATATACAATCACCAT2390.15172098574203624No Hit
GGTATGCTTATTAAGTATACTGTAACGCCATTACTTTCGATTCCAATGAAA2360.1498165382222617No Hit
TCCTAAACATGTTCAGTTCTTTTTTATTGTGTTTTGGGTATTATCGGTCCT2350.14918172238233687No Hit
CTCTTAGTTCTTTGTATAGGAGTGATGTTTTTGTTATTCATTCATGATCTA2310.14664245902263753No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2270.14410319566293817No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2250.1428335639830885No Hit
GATTATTATGGAACTGAAAAATTCCTATTGCCTAGTAATGTTGTAGCCATT2250.1428335639830885No Hit
GTAATAATCCAGATATACTCCACGCAGGACTTGTTAAATAGGTAGAAGAAA2240.14219874814316366No Hit
CTAAAAGAGTATAATTGGATTTGTTTGTAACACAGGGGATAAATGCTTGAG2190.1390246689435395No Hit
ATTCAGCTTTACCTTTTGTACAATTTGAAGTTGTAGCCTATATTTTTGCTT2160.13712022142376498No Hit
GTTAAATAATGGACTGAAACAATAAAAATTCCTTCTGGATGGATGTTTTAT2150.13648540558384015No Hit
TATCTAAATAAGGTGAATTAGGGTAATCCTTTATAAAGTTGCTGATTGCAA2140.13585058974391528No Hit
AAATTCCTAAGAAATGTTTCACGATTCTCTCATTCAGAGTAAGGTAGAGTT2110.13394614222414078No Hit
CATATTGATGAATAATAAAAAGCTCGAAGTAATCTTTGAAATACTGCAAGC2110.13394614222414078No Hit
TATTAGAATGAGGGGAATTGGTAAACTATTACAATCACTGAAAACAGCTAT2100.13331132638421594No Hit
ATATGAACATACATACTTCTTTACATTCCATAGCATTGTATCTGTCTCTTA2090.1326765105442911No Hit
GTTGTTTGTTTTCTTATTGTTGAGTTTTAAGAATTCTTTGCATATTTTGGA2080.13204169470436627No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2070.13140687886444144No Hit
AATTGTTGGTTATACATTTAAGTCACACAGAATTATGGTAAGAATAATGTC2070.13140687886444144No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.12823279966481724No Hit
CTGTTGTCTTAACATTTACTGTCAAAGGAAAAATGCCTTCTTGATACCATT1990.1263283521450427No Hit
TAGATATTTGTTTCCAATTTCTTCATCCATATGTAGGTGCAAATAACTAAT1950.12378908878534338No Hit
CATAAGAATAATTTAGATTCAACTTCATCGACAAATAATGAAAGTACGGAA1930.12251945710549371No Hit
GAAGTAGATTTTTCTTCCTCAAGCTGTCATTTATTTCCCTTTATCTATCCT1930.12251945710549371No Hit
TGCAATGATAGACGTAGAAAAAGAAGAAATTCAACATTATTTCCCGGAAAT1930.12251945710549371No Hit
ATTCCTAAGAAGAAATGATAAGATTGTTACGATCATGATTATGAGTACATA1910.12124982542564401No Hit
CTATAAAATCTCACAATCTACTACATTATGCTTTTCTAAAGACCCAAAGAA1910.12124982542564401No Hit
TAATGAAAAAGCAGAAATGTCTACATGCCCATCAACAGGGAAAAGGATAAA1900.12061500958571918No Hit
TTGCAAACCAGATAACAGTAATGGGATTTTTCATGTTTTCTTGGTGTAAAT1900.12061500958571918No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.12061500958571918No Hit
ACATCATATTGAGCAAAATGCATTTGAAGTAAGTGATAATTTCTATTTCAA1890.11998019374579434No Hit
ACAATACATTCACCTGTAACAAAAGTGGTTAAATGATCTCAAAATTCACTT1870.11871056206594467No Hit
CAGTTATTATAAACTTTGTGTCTGAGAACTCTAATATCTTGGTTAACTGTA1850.117440930386095No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1850.117440930386095No Hit
ATGTAGCTATAGTGTGATTTACAATAACTTTGTGTTTTTGTTATCAGCAAG1810.11490166702639565No Hit
ATCAAATACAGTGTGTATGCCAAGAATAATAATTGCATCAGTAATACCCTC1780.11299721950662114No Hit
CTATTACATTTACAAACTTACCAAAATGCTCAAAAACAGTAAGCCTATGAG1780.11299721950662114No Hit
CAGATGCACATTACTTTCATAGTAGAATTGGAAATTACTGGAATGTGGTTT1750.11109277198684661No Hit
GATTAAATCACCTCAAATGAATCTACTTGGTTCAATTTCAAAGTCTCAGAA1720.1091883244670721No Hit
TTTCCATTTAACTTAATTTCACCAAAAGGTTAAAGTAAGAAAGAGTAAAAC1720.1091883244670721No Hit
GTATTGTTATTAGGTGGCATTCAGTTAATGTCTATTGGTGTAGTTGGCGAA1720.1091883244670721No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1710.10855350862714727No Hit
AGGTTAATGAGAATTCCATTCTTGAATGTTTCATTAAAAATTCGGAGTTCC1710.10855350862714727No Hit
TATCAGATAATACTTGAGTAATCCTAGCCCTTCCTGAATCTGCATATTGCA1690.10728387694729757No Hit
GTCTTGTAAGTTGTAATATTGTGTTTTTTGTGTCAAACATTGCAAACCAGA1690.10728387694729757No Hit
GTAAAAAAGTTATTATTCAACACCACCATCAAACATGTTCGTTTGAATAGT1680.10664906110737274No Hit
GTCTCAGTTTTCCTTCTTTTTTTTGGGTTATTTTTTTTCCAATTGTTGTTG1660.10537942942752307No Hit
GACTAATCTTATTTTAAACAGCAGGTTAACATTTGTTTTTTCAGATTCATT1660.10537942942752307No Hit
AACATAGTCTGGGAACAATTGACATGTTCTAAATTTCAAAATTCCCTTTCA1650.10474461358759823No Hit
TTAGAGAACTTTATAAGCTACTAGAACGAGCTAAAAAGAAGTATCATAAGT1650.10474461358759823No Hit
AAGTTTCTAACACATAAGTTTTGGAGGACACATTCAAAACATAGCACTAAA1650.10474461358759823No Hit
TAACTAACGAGAAGTAAGATCTAAGATTTTTAAATGATAATGCCCTTGCAA1640.1041097977476734No Hit
TAATTATACTTTTGATTGGAAGAGAATGCTGTCTTTTGAAGGTAATACTGG1600.10157053438797405No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.10157053438797405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAG351.1951488E-745.0374951
ATGTCCC351.1951488E-745.0374955
GTATAGA253.8564096E-545.0374951
GGTTAGA253.8564096E-545.0374951
GTACGTA206.985995E-445.0374954
GAGCTAT206.9969444E-445.023196
GTACTAT206.9969444E-445.023196
AACGTAG207.018884E-444.99460634
AGATCGA207.018884E-444.99460618
ACTTGAG207.018884E-444.99460612
ACCGCAC800.044.99460640
TGGGTGA207.018884E-444.99460623
CGCATAT207.018884E-444.99460635
GAGAGTC406.7575456E-944.99460616
TAAGTAG207.018884E-444.99460639
CGATTAA207.018884E-444.99460635
TCGATTA207.018884E-444.99460634
TCTAGAC207.018884E-444.99460614
TCCTACA207.018884E-444.9946067
ATACTGG207.018884E-444.99460645
Produced by FastQC (version 0.11.2)