FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_69.351000000427c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_69.351000000427c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203863
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC2103010.315751264329476No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT36961.8129822478821562No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG17530.859891201444107No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG5130.2516395814836434No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4830.23692381648459993No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA4330.21239754148619414No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA3810.18689021548785215No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2590.127046104491742No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2570.12606505349180577No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC2230.10938718649288984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGCC207.021697E-444.99730319
TGCCGTC23800.044.61917545
ATGCCGT27650.038.40637644
TATGCCG29000.036.61849243
GTATGCC29200.036.44473342
CGTATGC29350.036.4117941
TATCTCG29050.036.4005736
CTCGTAT29150.036.35288239
ACTGTAT29300.036.32034732
GTATCTC29150.036.275735
TCGTATG29400.036.1202840
ATCTCGT29350.036.10516437
TGTATCT29350.036.02850734
TGCCTGA250.002103672835.9978422
TGATCTA250.002103672835.9978414
CTGTATC29650.035.9674933
AGACTCA29550.035.9369325
CTCAACT29550.035.9369328
AACTGTA29550.035.9369331
ACTCAAC29550.035.9369327
Produced by FastQC (version 0.11.2)