FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_68.351000000427af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_68.351000000427af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256700
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC33691.312426957537982No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT6120.23841059602649006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.10323334631865992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4050.044.44098345
TATGCCG4850.037.11050843
ATGCCGT4850.037.11050844
TAACGTA1050.036.4257330
GTATGCC4950.036.360842
CGTATGC5000.035.99719641
CACGACA1250.035.99719615
GAGACTC5050.035.64078524
TATCTCG5050.035.64078536
AGACTCA5100.035.29136725
CTCGTAT5100.035.29136739
CCACGAG5100.035.29136720
CCCACGA5150.034.94873419
GACTCAA5150.034.94873426
CTAACGT1100.034.7700229
TCTAACG1100.034.7700228
ATCTCGT5200.034.61268637
GACAAAC1300.034.61268618
ACACACG1300.034.61268612
CGAGACT5200.034.61268623
Produced by FastQC (version 0.11.2)