FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_66.3510000004276b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_66.3510000004276b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241042
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC100554.171472191568274No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT17090.7090050696559106No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC12300.5102845147318725No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5890.24435575542851454No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5720.23730304262327725No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5630.23356925349109284No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG5080.2107516532388546No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC4840.20079488221969613No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4700.1949867657918537No Hit
GATATATACAACTAGACATTTGTTGGGTTTTATTTAACTGTGTTGCTTTTG4570.18959351482314285No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG4170.17299889645787872No Hit
TGCTATAGCAGTGTTATTGAACAAAAGCAACACAGTTAAATAAAACCCAAC3110.12902315778992873No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC2960.12280017590295467No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC2920.12114071406642826No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA2910.12072584860729665No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA2880.11948125222990184No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC2720.11284340488379618No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA2550.10579069207855893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10850.043.34001545
ATAGGAC301.136012E-437.506923
ATGCCGT12650.037.17305844
CTCGCAT250.00210405135.9991739
CACGAGA13350.035.8980521
CCACGAG13400.035.76410320
TATGCCG13250.035.65955443
ACGAGAC13500.035.49918422
CTCGTAT13300.035.35632739
CGAGACT13500.035.3325223
TATCTCG13450.035.2965836
CGTATGC13400.035.2603841
CCCACGA13600.035.2381619
CTCAACT13500.035.16585528
GTATGCC13450.035.129342
ATCTCGT13550.035.03609537
ACTCAAC13650.034.94425227
GACTCAA13650.034.94425226
GAGACTC13700.034.81671524
TCTCGTA13650.034.7794238