FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_64.35100000042728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_64.35100000042728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148008
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC60164.064645154315983No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT46003.1079401113453327No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG5090.3439003297119074No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2180.14728933571158315No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1630.11012918220636722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6300.042.85360345
CGGGATA250.00210204735.99702516
ACTGGGA250.00210204735.9970258
TTGGAAC300.005118068330.0178053
TGATAGT602.3966822E-729.99752425
ACCTATG602.3966822E-729.9975247
CCTATGG602.3966822E-729.9975248
AGAGTGT406.131415E-428.1321816
AGTTAAT654.8287257E-727.69001819
TGTGTCG654.8287257E-727.69001831
TAACCTA709.168398E-725.7295465
ATGGATT709.2226765E-725.71216211
GTGTCGA709.2226765E-725.71216232
CTAAATC450.001217289525.0148373
CGAAACA651.5130972E-524.22876536
AATGATA851.8720493E-723.82156423
ATGCCGT11450.023.57883844
GTATGCC12100.022.87001242
TATGCCG12050.022.77820443
CGTATGC12200.022.68255441
Produced by FastQC (version 0.11.2)