FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_63.3510000004270e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_63.3510000004270e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224599
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC144656.4403670541721025No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT51112.275611200406057No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG7140.3178999016024114No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4830.21504993343692538No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4140.18432851437450745No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.13446186314275663No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.12288567624967163No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2540.11309044118629201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15200.044.55437545
CATACCC250.002103865635.99875310
ATGCCGT20650.032.79547544
GGACCGT352.8100974E-432.1489036
TATGCCG21500.031.49890943
CTCGTAT21650.031.2806739
GACCTGA21500.031.0803226
GTATGCC21800.031.06543542
CTGATTA21450.031.04787429
ATCTCGT21750.031.03340737
CGTATGC21850.030.99434941
AAATCTC21700.030.89754535
CCACGAG21750.030.82651720
CCTGATT21800.030.75581428
TCTCGTA22000.030.68075638
AATCTCG21950.030.6481436
TCGTATG22100.030.64373640
GAGACCT21900.030.61537624
ACGAGAC21900.030.61537622
ACCTGAT21800.030.54939827
Produced by FastQC (version 0.11.2)