FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_62.351000000426e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_62.351000000426e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335647
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC40441.204837224822507No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT18000.5362776965085343No Hit
GTATACACACAACACACGACAAACTATTCTAACGTATTCACACATTGACAG3690.10993692778424953No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACC253.8849597E-544.9998536
TGCCGTC5200.042.40370645
CGCGACA250.00210502235.9998822
CGGGGGA250.00210502235.9998836
CGTGGAA300.005142211429.999919
CCGGGGG300.005142211429.999935
ACCGGGG300.005142211429.999934
CATGCGG300.005142211429.999941
GCGCGAC300.005142211429.999921
GTAGCGG300.005142211429.99993
GGTGCTC300.005142211429.999919
ACCGTGC300.005142211429.999916
CGGTCTT300.005142211429.999945
CTAGGCT300.005142211429.99994
TCAACCG300.005142211429.99995
ATGCCGT7650.028.82343344
TATGCGC406.1570946E-428.12490718
CGTACCA406.1570946E-428.12490737
ATGCGCG406.1570946E-428.12490719
CTAACGT903.929017E-1027.4999129
Produced by FastQC (version 0.11.2)