FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_61.351000000426c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_61.351000000426c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65127
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC21913.364196109140602No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT1900.29173768176025305No Hit
CCATTAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG1670.256422067652433No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG1460.22417737651051023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.22264191502756153No Hit
GCCTCTAACCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTG1390.21342914612986932No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCTCA1270.19500360833448493No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1120.17197168609025443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.1673653016414083No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.16429437867551092No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.15968799422666483No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.15815253274371613No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA910.13972699494833174No Hit
ACCTCTGGGTTACAGGCAGGCGCGCTAACCACTACGCCACCTGTCTCTTAT860.1320496875335882No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCCCA850.1305142260506395No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT850.1305142260506395No Hit
CCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG830.1274433030847421No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA820.1259078416017934No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC800.12283691863589602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.11669507270410122No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGTCGTTAAGTGTCTTGCCCA700.10748230380640902No Hit
GTGGCGTAGTGGTTAGCGCGCCTGCCTGTAACCCAGAGGTCTGTCTCTTAT690.10594684232346033No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCA206.960807E-445.0307432
CTAGCAC206.960807E-445.0307433
TGCCGTC2800.041.78214645
ATGCCGT2800.041.78214644
ATTAAAT2850.041.0491332
GATTAAA2850.041.0491331
TTAAATC2850.041.0491333
GTATGCC2900.040.3413842
ATCTCGT2900.040.3413837
AAATCTC2900.040.3413835
TATGCCG2900.040.3413843
TGATTAA2900.040.3413830
CTGATTA2900.040.3413829
TAAATCT2950.039.65763534
CCACGAG2950.039.65763520
TCTCGTA2950.039.65763538
TCGTATG2950.039.65763540
GAGACCT3050.039.09502824
CCTGATT3000.038.9966728
CACGAGA3000.038.9966721
Produced by FastQC (version 0.11.2)