FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_60.351000000426a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_60.351000000426a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247846
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC6595826.6124932417711No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT125785.0749255586130095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG53532.159808913599574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC68500.044.66492545
GCGTTCC301.13833084E-437.4944513
ATGCCGT81650.037.47149344
ATACTCT250.002099205736.0164766
CCGGCGA250.002103370636.00193821
GTTCCCG250.002105455635.99467515
TATGCCG87350.035.0520543
GTATGCC87700.034.9378242
TCTCGTA88050.034.9266938
CGTATGC88200.034.89279641
ATCTCGT88150.034.88706237
CTCGTAT88200.034.8672939
CTGATTA87900.034.86535629
AAATCTC88100.034.85579335
TTAAATC88050.034.8244933
GATTAAA87850.034.8083631
CCTGATT88050.034.8059628
ATTAAAT88000.034.8001732
CGAGACC88550.034.7618923
TCGTATG88650.034.7410540
Produced by FastQC (version 0.11.2)