Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_1228_pb_6.35100000041ffa.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 28158 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC | 6669 | 23.684210526315788 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT | 657 | 2.3332623055614747 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG | 141 | 0.5007457916045174 | No Hit |
GAATAAAATACTTAGGAACAAATTTAATGAAAGCAGCGCATACAAGACTTG | 47 | 0.16691526386817246 | No Hit |
ACACATTTTTTATTCAAAGTTTTCCAACATCGGTAATCTCGAAAAATCAAA | 46 | 0.1633638752752326 | No Hit |
ACATTCATTATAATCTGGCTGAATTAATTGCTGAAGCAAAGGATAAAAATA | 33 | 0.1171958235670147 | No Hit |
GATAAACATATCTTTCGAGGAGTTTTATCTAGTATACGTACATTCTTCTCT | 33 | 0.1171958235670147 | No Hit |
ATATATACTTTTGGCTTTAGGAAAAGATGAGCAGTCCAGATTTCATATGCA | 32 | 0.11364443497407486 | No Hit |
TTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTCT | 32 | 0.11364443497407486 | No Hit |
ACAAATATTAGATGAAACAAGACAAAAGGGCATGTTCATACTAACAGGCTC | 32 | 0.11364443497407486 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG | 31 | 0.11009304638113501 | No Hit |
ATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTCTTC | 29 | 0.10299026919525535 | Illumina PCR Primer Index 10 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 765 | 0.0 | 42.647057 | 45 |
ATGCCGT | 845 | 0.0 | 38.343193 | 44 |
TCGTATG | 875 | 0.0 | 37.285713 | 40 |
GTATGCC | 870 | 0.0 | 37.24138 | 42 |
TATGCCG | 870 | 0.0 | 37.24138 | 43 |
AGCCCAC | 885 | 0.0 | 37.118645 | 17 |
CGTTATC | 885 | 0.0 | 37.118645 | 33 |
GCCCACG | 885 | 0.0 | 37.118645 | 18 |
CCACGAG | 885 | 0.0 | 37.118645 | 20 |
ACGAGAC | 885 | 0.0 | 37.118645 | 22 |
TATCTCG | 885 | 0.0 | 37.118645 | 36 |
GCGTTAT | 885 | 0.0 | 37.118645 | 32 |
CGAGCCC | 885 | 0.0 | 37.118645 | 15 |
TCTCCGA | 885 | 0.0 | 37.118645 | 11 |
CGAGACA | 885 | 0.0 | 37.118645 | 23 |
GTTATCT | 885 | 0.0 | 37.118645 | 34 |
CTCCGAG | 885 | 0.0 | 37.118645 | 12 |
CAATGCG | 880 | 0.0 | 37.073864 | 28 |
TGCGTTA | 880 | 0.0 | 37.073864 | 31 |
ATGCGTT | 880 | 0.0 | 37.073864 | 30 |