FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_6.35100000041ffa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_6.35100000041ffa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28158
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC666923.684210526315788No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT6572.3332623055614747No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG1410.5007457916045174No Hit
GAATAAAATACTTAGGAACAAATTTAATGAAAGCAGCGCATACAAGACTTG470.16691526386817246No Hit
ACACATTTTTTATTCAAAGTTTTCCAACATCGGTAATCTCGAAAAATCAAA460.1633638752752326No Hit
ACATTCATTATAATCTGGCTGAATTAATTGCTGAAGCAAAGGATAAAAATA330.1171958235670147No Hit
GATAAACATATCTTTCGAGGAGTTTTATCTAGTATACGTACATTCTTCTCT330.1171958235670147No Hit
ATATATACTTTTGGCTTTAGGAAAAGATGAGCAGTCCAGATTTCATATGCA320.11364443497407486No Hit
TTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTCT320.11364443497407486No Hit
ACAAATATTAGATGAAACAAGACAAAAGGGCATGTTCATACTAACAGGCTC320.11364443497407486No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG310.11009304638113501No Hit
ATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTCTTC290.10299026919525535Illumina PCR Primer Index 10 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7650.042.64705745
ATGCCGT8450.038.34319344
TCGTATG8750.037.28571340
GTATGCC8700.037.2413842
TATGCCG8700.037.2413843
AGCCCAC8850.037.11864517
CGTTATC8850.037.11864533
GCCCACG8850.037.11864518
CCACGAG8850.037.11864520
ACGAGAC8850.037.11864522
TATCTCG8850.037.11864536
GCGTTAT8850.037.11864532
CGAGCCC8850.037.11864515
TCTCCGA8850.037.11864511
CGAGACA8850.037.11864523
GTTATCT8850.037.11864534
CTCCGAG8850.037.11864512
CAATGCG8800.037.07386428
TGCGTTA8800.037.07386431
ATGCGTT8800.037.07386430