FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_56.35100000042629.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_56.35100000042629.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208841
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC21731.0405044986377197No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT7890.37779937847453327No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG5010.23989542283363896No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA3390.16232444778563596No Hit
AAGGAATGTGACAATAACGCAAGAAACCTGTCTCTTATACACATCTCCGAG2890.13838278882020294No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA2160.1034279667306707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGAG207.02057E-444.999287
CCTGAGT207.02057E-444.999288
TGCCGTC2050.041.7066545
TCCGTGG250.00210086336.008052
CTGAGTC250.00210333535.9994249
CCGTGGA300.005132114530.0067063
GTCTACG300.005132114530.0067061
CTACGGT300.005132114530.0067063
GTCGTAT300.005132114530.0067061
GAGTCTC300.0051381329.99952311
CCCCCTG300.0051381329.99952334
AGAGGGT300.0051381329.9995238
AGAGGAT300.0051381329.99952334
TCGGTCT300.0051381329.99952315
GTGCTAA702.8561772E-828.935041
GTTTTAT554.136311E-628.6427651
TTAATGC406.1496964E-428.1245529
TTATATC406.1496964E-428.124554
ATGCCGT3100.027.58020644
TATTGTA450.001221601225.0055892
Produced by FastQC (version 0.11.2)