H23LNBCXX_l02n01_bir_1228_pb_55.3510000004261c.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pb_55.3510000004261c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49068
Sequences flagged as poor quality	0
Sequence length	51
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	369	0.752017608217168	No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT	218	0.44428140539659255	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC	163	0.3321920599983696	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG	136	0.27716638134833294	No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCCTGTCTCTT	133	0.2710524170538844	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA	131	0.2669764408575854	No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT	127	0.25882448846498735	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGCTGTCTCTT	120	0.2445585717779408	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT	108	0.22010271460014674	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC	103	0.20991277410939918	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC	98	0.19972283361865167	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC	84	0.17119100024455858	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG	81	0.16507703595011006	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA	78	0.15896307165566154	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATT	77	0.15692508355751203	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT	76	0.15488709545936252	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG	71	0.144697154968615	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA	68	0.13858319067416647	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	66	0.13450721447786745	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC	65	0.13246922637971795	No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGG	64	0.13043123828156844	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA	62	0.12635526208526943	No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT	59	0.12024129779082089	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCG	56	0.11412733349637239	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC	54	0.11005135730007337	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC	51	0.10393739300562485	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGCTGTCTCTT	50	0.10189940490747534	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	70	0.0	41.780033	45
GTATGCC	75	0.0	38.994698	42
ATCTCGT	75	0.0	38.994698	37
TATGCCG	75	0.0	38.994698	43
TCGTATG	75	0.0	38.994698	40
CTCGTAT	75	0.0	38.994698	39
TAGTGGT	35	6.1326027E-6	38.566185	7
GTATCTC	70	0.0	38.566185	35
TCGTGTA	70	0.0	38.566185	31
TATCTCG	70	0.0	38.566185	36
ATCGTGT	70	0.0	38.566185	30
CGATCGT	70	0.0	38.566185	28
CACGAGA	80	0.0	36.55753	21
CCCACGA	80	0.0	36.55753	19
TACGGGT	25	0.0020784866	36.031837	2
TAGCGCG	25	0.002088938	35.995106	14
GTGTATC	75	1.8189894E-12	35.995106	33
GATCGTG	75	1.8189894E-12	35.995106	29
AGACGAT	75	1.8189894E-12	35.995106	25
ACGATCG	75	1.8189894E-12	35.995106	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content