FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_54.351000000425f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_54.351000000425f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46341
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC25395.478949526337368No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT10782.3262337886536764No Hit
GTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGAAACTACATCTCT3850.8307977816620271No Hit
GTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGAAACTACATCT2710.5847953216374269No Hit
GTATGTGTCGGCTCCATCTTCTCATTGAGCAATTGGTGAAGAGATGTAGTT2470.5330053300533005No Hit
GTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGAAACTACAT1950.42079368162102676No Hit
GTATGTGTCGGCTCCATCTTCTCATTGAGCAACTGGTGAAGAGATGTAGTT1460.31505578213676877No Hit
AATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATG1350.2913187026607108No Hit
GGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGACACCTT1330.2870028700287003No Hit
TATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGA1190.25679204160462654No Hit
GAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGACACCTTACTACA1020.22010746423253708No Hit
CCTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGAT840.1812649705444423No Hit
GAGCAACTGGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATG770.16615955633240545No Hit
GAGCAATTGGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATG720.1553699747523791No Hit
ATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGG690.1488962258043633No Hit
CTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATA650.14026456054034223No Hit
ATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACA640.13810664422433697No Hit
GATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGACACCTTACTACACA640.13810664422433697No Hit
GCAACTGGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGA630.1359487279083317No Hit
CCCTTAACCTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAAC630.1359487279083317No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA610.1316328952763212No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT610.1316328952763212No Hit
ATTGCATTGTATGTGTCGGCTCCATCTTCTCATTGAGCAATTGGTGAAGAG610.1316328952763212No Hit
CTGGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGACACC560.12084331369629486No Hit
ACCTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGA510.11005373211626854No Hit
CTTTAACCCTTAACCTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGA510.11005373211626854No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGG206.963294E-445.06
TATAGTG206.963294E-445.05
TGCCGTC3200.043.5937545
GCCTGTA301.12308975E-437.50000424
ATGCCGT4300.032.44186444
GCCCACG4450.031.34831618
CCACGAG4550.031.15384520
TATGCCG4550.031.15384543
CCCACGA4550.031.15384519
TCGTATG4550.031.15384540
AGCCCAC4500.031.017
GATCGTG4500.031.029
ATCGTGT4500.031.030
TCGTGTA4450.030.84269731
ACGATCG4450.030.84269727
GTATGCC4600.030.8152242
CACGAGA4600.030.8152221
CGTATGC4600.030.8152241
GTGTATC4500.030.533
TATCTCG4500.030.536
Produced by FastQC (version 0.11.2)