FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_53.351000000425ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_53.351000000425ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207945
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC9140.4395393012575441No Hit
CCATTAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG8710.41886075645002285No Hit
GCCTCTAACCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTG6230.29959845151362136No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCCCA4620.22217413258313495No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCTCA4570.21976965062877205No Hit
GTGGCGTAGTGGTTAGCGCGCCTGCCTGTAACCCAGAGGTCTGTCTCTTAT4530.2178460650652817No Hit
ACCTCTGGGTTACAGGCAGGCGCGCTAACCACTACGCCACCTGTCTCTTAT4320.20774724085695734No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3230.15532953425184545No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGTCGTTAAGTGTCTTGCCCA3100.14907788117050183No Hit
CCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG2970.14282622808915818No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC2740.1317656110990887No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA2630.12647575079949025No Hit
AGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCCCAAGGACAC2280.10964437711894971No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT2200.10579720599196903No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2100.10098824208324318No Hit
GTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATGTGTCCTTGGGCAAG2080.100026449301498No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC207.0230884E-444.99591429
TGCCGTC1150.044.9959145
GCGACGG403.442492E-739.37142612
GACGGAT403.442492E-739.37142614
ATGCCGT1400.036.96092644
GGATAAG250.002096644436.0227171
TTATCTA250.002104087735.9967328
TGTCTGC458.6605723E-734.99682229
ACGGATC458.6605723E-734.99682215
ATCGTGT1550.033.38406430
GTATGCC1550.033.38406442
CGATCGT1550.033.38406428
CTCGTAT1500.032.99739
TATCTCG1500.032.99736
GGATCAT551.12106136E-732.724317
GATCATT551.12106136E-732.724318
TATGCCG1600.032.34081343
GTGTATC1600.032.34081333
ATATGTG352.8020903E-432.1631435
TCTAGCA708.0035534E-1032.1631432
Produced by FastQC (version 0.11.2)