FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_52.351000000425a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_52.351000000425a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217471
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC16730.7692979753622322No Hit
CCATTAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG3920.18025391891332637No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT3820.17565560465533336No Hit
CATTAAATCAGTTATGGTTCATTTGATCGTATGGTTTACTTGGATAACTGT3790.17427611037793544No Hit
TCTTAATATAGGTCAATATATGAATGCTCTCTTTACAGTCTGTCTCTTATA3560.1636999875845515No Hit
CTAATGGGTTGTCTAAATTGTAAGCCATATCAAATAAAATCATACACAAAG3480.1600213361781571No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCTCA3190.14668622482997734No Hit
GCCTCTAACCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTG2870.13197161920439968No Hit
ACTGTAAAGAGAGCATTCATATATTGACCTATATTAAGACTGTCTCTTATA2860.13151178777860037No Hit
CTTCTCTATTAAATAGGGTAAAAGTAGTTGAAAACTTAAACGAGGTTAATA2640.12139549641101573No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCCCA2460.1131185307466283No Hit
GAAATATCCTATTAACCTCGTTTAAGTTTTCAACTACTTTTACCCTATTTA2240.10300223937904365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGG207.0218975E-444.99839413
TCGCTTG207.0218975E-444.99839444
TGCCGTC1700.043.67490445
ATAGAGC403.4419645E-739.37359217
TCTTAGT301.1371197E-437.4986639
ATGCCGT2100.035.35587744
TAGAGCA458.659281E-734.9987518
GCGATAG458.659281E-734.9987514
GATAGAG458.659281E-734.9987516
CACGAGA2350.032.55202521
AGACGAT2350.032.55202525
GAGACGA2350.032.55202524
CGAGACG2350.032.55202523
CCACGAG2350.032.55202520
GTACCAT352.809706E-432.1490976
GGGCTTC352.8135016E-432.1417126
TACCGGG352.8135016E-432.1417121
TATGCCG2250.031.99885643
GACCAGA501.9724157E-631.49887541
GTATGCC2300.031.30322842
Produced by FastQC (version 0.11.2)