FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_5.35100000041fc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_5.35100000041fc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438550
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC100862.299851784289135No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT14180.32333827385702885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.13795462319005813No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4960.11309998859879147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9750.042.92003345
ATGCCGT10800.038.7472544
TATGCCG11250.037.1973643
GCGTTAT11400.036.9052832
CTCGTAT11450.036.74411839
ATCTCGT11700.036.5358737
CCACGAG11650.036.49955420
CGTTATC11600.036.4629333
ATGCGTT11550.036.42598730
CAATGCG11550.036.42598728
CCCACGA11700.036.34357519
CGAGACA11700.036.34357523
ACGAGAC11800.036.03557622
TGCGTTA11700.035.9589931
GTATGCC11700.035.76669342
AATGCGT11800.035.65424729
GAGACAA11900.035.54369724
CACGAGA12000.035.43498621
GTTATCT11950.035.39497834
CGTATGC11900.035.35463341
Produced by FastQC (version 0.11.2)