FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_48.35100000042537.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_48.35100000042537.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156340
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC9010.5763080465651784No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT7130.4560573109888704No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG2590.16566457720353076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG1100.042.95454424
GCCGTCT1300.041.53846416
TGCTTGA1200.037.50000425
CCGTCTT1450.037.2413817
GTACTAG250.00210155536.01
CGTACGG250.00210155536.023
ACGTACG250.00210155536.022
GCTTGAA1300.034.61538726
CGTCTTC1600.033.75000418
CCTCTTA401.5519887E-533.7500048
CTTCTGC1650.032.72727221
CCTACAC352.809313E-432.142863
CCGACAA352.809313E-432.142869
TCTGCTT1550.031.93548423
ATCTATA300.0051338230.0000021
CGACATA300.0051338230.00000227
CGACAAT300.0051338230.00000210
ACCTCTT300.0051338230.0000027
TAACCGT300.0051338230.00000210
CGTCGGA300.0051338230.00000228
Produced by FastQC (version 0.11.2)