FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_47.35100000042500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_47.35100000042500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112709
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC1381612.258116033324756No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG75786.723509213993559No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT38203.389259065380759No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA2040.1809970809784489No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGC1840.16325226911781668No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1840.16325226911781668No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACATCTCCGA1710.15171814140840573Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA1680.14905641962931088Illumina PCR Primer Index 8 (96% over 27bp)
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC1260.11179231472198317No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTC1230.10913059294288831No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACATCTCCG1230.10913059294288831Illumina PCR Primer Index 8 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT207.007226E-444.99867234
AGCAGGG207.007226E-444.9986727
ATCGCCC207.007226E-444.99867233
CGTCTTC18850.044.6405918
GCCGTCT18800.044.63963716
GCTTGAA17750.044.49164226
CCGTCTT18950.044.4050217
TCTTCTG18850.044.28251320
CTGCTTG18150.044.2548924
GTCTTCT19150.044.05874619
CTTGAAA18050.044.00146527
TTCTGCT18650.043.9129122
CTTCTGC19050.043.817621
TCTGCTT18750.043.79870223
TGAAAAA18500.043.6608729
TGCTTGA18100.043.63130625
TTGAAAA18200.043.51519428
GAAAAAA18600.043.4261430
TGCTTGG301.1335986E-437.4988925
CTCAGTG301.1335986E-437.498899
Produced by FastQC (version 0.11.2)