FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_44.351000000424be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_44.351000000424be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260412
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT72492.783665883292629No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC60532.324393653134264No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG24290.9327527149286516No Hit
AAAAAGGCTTTGGAAAATATCAACTCCCGTCTTCGTCTTGTTATGAAGAGT4340.1666589865290386No Hit
AAGGAATGTGACAATAACGCAAGAAACCTGTCTCTTATACACATCTCCGAG3390.13017833279572372No Hit
GTTTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAAACCATAC2760.10598589926731486No Hit
GTATACACACAACACACGACAAACTATTCTAACGTATTCACACATTGACAG2720.1044498717417016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT5300.044.5720516
CCGTCTT5850.040.76609817
CTGCTTG5550.040.1320524
TCTGCTT5900.038.8953223
GCTTGAA5700.038.6812426
CTTCTGC6050.038.67471321
TGCTTGA5800.038.0143225
CGTCTTC6300.037.4971218
ACGCAAG850.037.05597717
TTCTGCT6300.036.42577422
CTGTAGC250.00210491735.9972349
GACCGTG250.00210491735.9972347
CGACACG250.00210491735.99723420
CGCAAGA900.034.9973118
AATAACG1000.033.74740613
CTTGAAA6800.033.41655327
TGAAAAA6700.033.24371329
TAACGTA754.7293724E-1132.99746330
GTATAGA352.803027E-432.1650921
CGCGGTT352.8156975E-432.1403929
Produced by FastQC (version 0.11.2)