FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_43.35100000042495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_43.35100000042495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141804
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC23801.6783729655016784No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT6000.42311923500042314No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2450.17277368762517278No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG2190.15443852077515444No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1850.13046176412513044No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1650.11635778962511636No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCTT1550.042.09347523
GCCGTCT1650.040.90588416
CCGTCTT1700.039.7027717
CGTCTTC1850.036.4836318
TATCGAT250.002101706835.99717730
GCTTGAA1850.034.05138826
AGCCATA401.5518082E-533.74735323
CTGCTTG1950.033.45891624
CTTGAAA2000.032.62244427
GTCTTCT2100.032.1403419
TGCTTGA2000.031.4975325
CTTCTGC2150.031.39288921
ATCGATG300.005134199329.99764831
TGAAAAA2200.029.65676729
GATATAA3850.029.2184930
TTCTGCT2250.028.99772822
GACAGAT3900.028.84389526
AGATATA3900.028.84389529
CCCACGA3900.028.84389519
CGAGACA3950.028.4787823
Produced by FastQC (version 0.11.2)