FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_42.3510000004246e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_42.3510000004246e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303608
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC87192.871795209612395No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT22890.7539327026955811No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT16100.530289056941846No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA14020.4617796632499802No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT13800.4545334773787252No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC13800.4545334773787252No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG11010.36263866564780906No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA10990.3619799214776949No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT10450.3441938288846144No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA10030.3303602013122184Illumina PCR Primer Index 8 (96% over 27bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC9250.3046691786777687No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC9070.29874048114674184No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT8810.2901768069352586No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA8770.28885931859503045No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT8760.2885299465099734No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT8530.2809543885536613No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT8490.2796369002134331No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC8440.27799003978814785No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT8050.265144528470923No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT7880.2595452030249532No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC7050.23220731996521832No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA6990.23023108745487603No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA6920.22792548285947672No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT6840.2252905061790203No Hit
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACA6600.2173855761376512No Hit
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCTGTCTCTTATACAC6210.20454006482042633No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA6100.20091697188479885No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC6080.20025822771468474No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA5920.19498827435377197No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5750.18938894890780217No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA5570.18346025137677532No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5500.18115464678137597No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA5480.1804959026112619No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT5420.1785196701009196No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTA5390.17753155384574848No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC5290.174237832995178No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT5260.17324971674000686No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5180.1706147400595505No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACCTGTCTCT5050.16633290295380887No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5030.16567415878369476No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCT5020.16534478669863772No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG5020.16534478669863772No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA4880.16073357750783904No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4810.1584279729124397No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC4660.153487391636584No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTA4640.15282864746646993No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA4490.14788806619061423No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATCTGTCTCTTAT4480.14755869410555716No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC4470.1472293220205001No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4440.14624120576532898No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4420.14558246159521487No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC4410.14525308951015783No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4400.1449237174251008No Hit
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACTGTCTCTTATACACA4370.14393560116992965No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC4350.14327685699981554No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATC4320.1422887407446444No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTATAC4270.14064188031935917No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACACA4170.1373481594687887No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG4120.13570129904350348No Hit
TTTTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3980.1310900898527048No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT3920.12911385734236253No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTCC3860.12713762483202024No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAA3750.1235145318963927Illumina PCR Primer Index 8 (96% over 27bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACT3710.12219704355616454No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3560.11725646228030882No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3560.11725646228030882No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA3530.11626834602513768No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG3520.11593897394008063No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTC3500.11528022976996653No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATACA3490.11495085768490948No Hit
AAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT3470.11429211351479539No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCCTGTCTCTT3460.11396274142973836No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC3440.11330399725962424No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3350.11033964849411083No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACTGTCTCTT3310.10902216015388264No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTA3260.1073752997285974No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA3260.1073752997285974No Hit
AAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACCTGTCTCTTA3250.10704592764354036No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTCCG3230.10638718347342627No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCCTGTCTCTTATACACATC3220.10605781138836921No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCACTGTCTCTTATACACATC3210.10572843930331216No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACCTGTCTCTTATACACATCTC3160.10408157887802692No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA3140.10342283470791283No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCT3140.10342283470791283No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT3110.10243471845274169No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT3110.10243471845274169No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG253.880814E-545.006262
GTCCGTA253.8845916E-544.99884413
CACGTCT207.0259656E-444.99884423
CGTCCGT253.8845916E-544.99884412
CCGTCTT18550.044.75626817
GCCGTCT18550.044.75626816
CGTCTTC18450.044.75495518
GCTTGAA17650.044.74389626
CTTGAAA17650.044.74389627
TTGAAAA17700.044.49038728
GTCTTCT18600.044.39402419
CTTCTGC18800.044.04142421
TGAAAAA18500.043.7826629
TTCTGCT18550.043.30077722
TCTGCTT18650.043.1892423
TCTTCTG19150.043.11899620
GAAAAAA19400.041.75150730
TTCTGCC603.6379788E-1241.24894322
GCCTGAA556.002665E-1140.9080426
TGCCTGA556.002665E-1140.9080425
Produced by FastQC (version 0.11.2)