FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_40.3510000004242b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_40.3510000004242b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125005
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC3022824.181432742690294No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG106828.545258189672413No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT97147.770889164433423No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGC4750.3799848006079757No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTC1880.15039398424063039No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG207.009159E-445.011
GGTGTAC207.009159E-445.037
GGGCTAA207.009159E-445.07
GGGGCTA207.009159E-445.06
TGCCGTC30500.044.85245545
TAGGGGC301.1341114E-437.4999964
ATAAAGA301.1341114E-437.4999963
TAATGTA301.1341114E-437.49999611
TTAGGGG250.002099947536.03
ACTATAT250.002099947536.07
CAAGTCA250.002099947536.015
ATTACGT250.002099947536.016
ATTCGTG250.002099947536.07
ACTTACG250.002099947536.035
GGGGTTA250.002099947536.035
TCCAATT250.002099947536.019
TTAAAGC250.002099947536.033
AGGGGCT250.002099947536.05
GTAAGTG250.002099947536.05
ATGCCGT41800.032.781144
Produced by FastQC (version 0.11.2)