FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_39.3510000004241e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_39.3510000004241e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169842
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC7651445.05010539207028No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT1894411.15389597390516No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG112736.637345297394049No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACT207.016295E-445.04
TGCCGTC78250.044.76996645
CACCCGT301.1358289E-437.50000411
TGTTGGT250.00210206636.02
TGTTGCT250.00210206636.033
GTCACCA250.00210206636.04
TCCACTT250.00210206636.030
CCACCCG250.00210206636.010
ATGCCGT98050.035.68332344
GGGAAGC551.1181146E-732.72727218
GACCTCC352.81033E-432.1428626
TACTTAT111050.031.74921232
TATCTCG111000.031.74324436
CTTATCT110950.031.73726834
ACTTATC111100.031.73492433
CTACTTA110900.031.73128931
TTATCTC111100.031.6944235
TCTACTT110750.031.69300330
TATGCCG110400.031.69157643
ATCTCGT111300.031.65768237
Produced by FastQC (version 0.11.2)