FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_38.351000000423e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_38.351000000423e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163662
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC10426463.70690813994696No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT1865311.397269983258179No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG83475.1001454216617175No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCTGTC2470.1509208001857487No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCAGTC1700.10387261551245859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC108450.044.6680545
GTATGCT451.9110303E-840.00000442
GAGACCA451.9110303E-840.00000424
GCCGTCT750.039.00000445
ATGCCGT129300.037.43039344
CTATACA250.002101843536.02
TATGCAG250.002101843536.043
CTTATTT250.002101843536.034
ATGCAGT250.002101843536.044
CGAGACT250.002101843536.023
TGCAGTC250.002101843536.045
TCTCGTA138500.035.12274638
AGCCCAC139050.035.11326617
CTCGTAT138550.035.1100739
ACGAGAC139200.035.1077622
CGTATGC138700.035.1045441
TATGCCG138000.035.1032643
ATCTCGT138600.035.097437
TCGTATG138750.035.09189240
CACGAGA139200.035.09159521
Produced by FastQC (version 0.11.2)