FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_36.351000000423a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_36.351000000423a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC74.093567251461988No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT31.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC21.1695906432748537No Hit
ATTAGGGCCACATAGCCCACAAAATGGACACCCCCGCCTGTCTCTTATACA10.5847953216374269No Hit
CCTTGATGAAGATTGAAAGAACCATTTGCAGACCAAATGCTGCAGCCATTA10.5847953216374269No Hit
GTGAAAGTGAGATATTCGCTTGGATTCAGACGATATGTATTGATCATCTGT10.5847953216374269No Hit
GGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGC10.5847953216374269No Hit
TTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG10.5847953216374269No Hit
AACTATATCCATAATAGCAGCAAAAAATAGGACCACAAATATTTCACCAAA10.5847953216374269No Hit
AAAAAGTACTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCT10.5847953216374269No Hit
GCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG10.5847953216374269No Hit
ATCTTCTACGATGGTTTAGATCTTTTCAAAAAAGTAAGCCTCTCCGATTCA10.5847953216374269No Hit
AGAAAGAATAAACAAGCAAGGGGTGCTGTGTTTAATAATGTGAACCATGAT10.5847953216374269No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCCACTTATCTCGTATGCCGTC10.5847953216374269No Hit
TAATTTCTCTCAATGGAATCGTTTCAAATTGTTTAACTTTTGTGGGTACTT10.5847953216374269No Hit
GTACGGGAGGTGATAGAAAGAGAAAGGGACAAATGATTCAGTTCTAAATGA10.5847953216374269No Hit
GAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTACC10.5847953216374269No Hit
GCTTGGAGGAGAGGAAGATTGGCAGGTAAGAAGAAAGGACATTGGATGAGG10.5847953216374269No Hit
AAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGTGCA10.5847953216374269No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.5847953216374269No Hit
GACATAATTCTTTTGCAAGATCATAATGATATTGCGCCGGTTTTGTATAAT10.5847953216374269No Hit
AAAGAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC10.5847953216374269No Hit
CAACAGAAGACAACGTTACATGTCTTGAGAGAGACAAGTATGCTTTGATAA10.5847953216374269No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTATTCTTATCTCGTATGCCGT10.5847953216374269No Hit
CATAAGGGCTCATCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10.5847953216374269RNA PCR Primer, Index 38 (96% over 26bp)
GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATA10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGTCCAATCTCGTATGCCGTC10.5847953216374269RNA PCR Primer, Index 13 (95% over 21bp)
GGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAA10.5847953216374269No Hit
GCTTTTGAAGGTAACACTCTCTGTGGGAACTGTCTCTTATACACATCTCCG10.5847953216374269No Hit
TTATAGTTCGGTTTGAGAATTTTATGGGAAAATGTCTGCAGACTTAGCTGA10.5847953216374269No Hit
AATAATGCTAATGCCTTAGTTCTGCCTCATTAATCCTGTCTCTTATACACA10.5847953216374269No Hit
GTTACCCGGTTGGTGATATCACCAAGGGAATGGATGAAGCTGAACATAAGA10.5847953216374269No Hit
ATGTTAAATTTCTTGCTGAAGAAGTGGTGTATGATCTGTCTCTTATACACA10.5847953216374269No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTCTGTCTCTTATA10.5847953216374269No Hit
CTCAAAGATCGAATCGACCAAACAATCGGGAAGATAATAGCACAGCAACAG10.5847953216374269No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCCTGTCTCTTATACA10.5847953216374269No Hit
AAAGTACTCTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC10.5847953216374269No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTATT10.5847953216374269No Hit
GTATCAACGCAGAGTACGGGAACCAGTGGTATCAACGCAGAGTCTGTCCCT10.5847953216374269No Hit
ACGAAATAGCTTCCATTCAGCGGCACAAGGAAAAACGATTGAGAAAATCTC10.5847953216374269No Hit
GGTAAACGTAAGAAGCATTACATATCCGCAAATGTTAATAAACGCAAGAAA10.5847953216374269No Hit
CCTTGAAAGAGTCTCATTGCTAATGCTCTTAGACCTGTCTCTTATACACAT10.5847953216374269No Hit
GGTATCAACGCAGAGTACGGGCTGTCTCTTATACACATCTCCGAGCCCACG10.5847953216374269No Hit
ATACCAAAGAGAAGTTTTGAGAAAACTTAGAATAGTCATCACCATGGCTGT10.5847953216374269No Hit
GGTATCAACGCAGAGTACGGGAGCAGTGGTATCTGTCTCTTATACACATCT10.5847953216374269No Hit
GAACAATGTTTCGTGTGCACTTGAGGAAAATGTGTATTTTACTGCTGTCTC10.5847953216374269No Hit
CATTACTCCTTGATTCTCTTCTTCAACTGTTTTCACACTGTCTCTTATACA10.5847953216374269No Hit
GGCTAATACCTGAGCATGTGAGACGACAAATGAGCACAGGATTACAAAACA10.5847953216374269No Hit
GTACGGTGGCTTAATGCTGGGTATAATGGTTGCCTCATCAGAGACTGTCTC10.5847953216374269No Hit
CTCTTCATCTCTGTGCCGTACTCATTGTACCGTTTCCCAAAAGCTTGTCTA10.5847953216374269No Hit
CACCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTC10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC10.5847953216374269RNA PCR Primer, Index 13 (95% over 21bp)
ATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTCC10.5847953216374269No Hit
GCCAAAATGTCATAACACAACAAAATGGACAATAAGATTATTAAAAACGAA10.5847953216374269No Hit
TCTTATACACATCTCCGAGCCCACGAGTGTTATACATCTCGTATGCCGTCT10.5847953216374269RNA PCR Primer, Index 39 (95% over 22bp)
TATCAACGCAGAGTACGGGGAATTCGACGTAATTCTGAAAGCCTGTCTCTT10.5847953216374269No Hit
AAATAGGAGACTTTGTTGCGGAAAAAGACCTGTCTCTTATACACATCTCCG10.5847953216374269No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACCCGTACCCTTC10.5847953216374269No Hit
GAGATAGGAGGAGGAAAATGCTAAGAGAGTGATAGAGCTGTCTCTTATACA10.5847953216374269No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAACTGTCTCTTAT10.5847953216374269No Hit
CTCTAACAGATCTTGTGTTGTCTTTAAGTTTTTGCTTGCAATGTTCCCTGT10.5847953216374269No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT10.5847953216374269No Hit
GCTACAGTCACTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCCGTC10.5847953216374269TruSeq Adapter, Index 16 (96% over 28bp)
GAATTTAGCATCCTTATCCTTTCTGTTCCTCTCAAGATGCTGTCCCTTATA10.5847953216374269No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACCGA10.5847953216374269Illumina PCR Primer Index 5 (96% over 28bp)
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10.5847953216374269No Hit
GGACGTGTGAGAACCCTTAATCCAATTTTCACCATCTGAAGATCTGTCTCT10.5847953216374269No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATATGCCTCA10.5847953216374269Illumina PCR Primer Index 8 (96% over 27bp)
AAAAAGTACTCTGCGTTGCTACCACCCCGTACCTGTCTCTTATACACATCC10.5847953216374269No Hit
CTTCTTGCATTTGATGGTGTTCCTATAGCAAATGATGGAACAGGGAGAGAA10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTACTAATCTCGTATGCCGTC10.5847953216374269RNA PCR Primer, Index 29 (95% over 21bp)
GAAACCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG10.5847953216374269No Hit
GTGCGACAGGGTCCCGGGCACGACGGGGCTCTCACCCTCCTGTCTCTTATA10.5847953216374269No Hit
GTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTGA10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCTTATCTCGTATGCCGTC10.5847953216374269RNA PCR Primer, Index 27 (95% over 21bp)
GTCTAATGAAGCTTTTGGTTCAGGTCATTTAGAAAATTAAGGGCCTGGATA10.5847953216374269No Hit
CAATAATACCAGGAAGATCTAATAACTGGATTTTAGCTCCTGTCTCTTATA10.5847953216374269No Hit
TAAGGTCATTGCTAATGCCGCTATGAGAGCCAAAATCAAAGAATCTATGGG10.5847953216374269No Hit
CTCTAAGAAGATTCAGGCTCTCCCTAGTCATGAAGCTGTCTCTTATACACA10.5847953216374269No Hit
CTTACATACCAGTCAAGGGTGATCTTCAAGCCTTATCTCAAGGTTACATTG10.5847953216374269No Hit
CTCCTTGAAGAGGTCCTTCATGTTCCTTGTAAGTTGGATCTGTCTCTTATA10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTACTGATCTCGTATGCCGTC10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTATTTATCTCGTATGCCGTC10.5847953216374269No Hit
GTCATGGAAAGTTATTGTTCACCTTCCTTTTTTGAATTCTCCCTTTAGACC10.5847953216374269No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA10.5847953216374269No Hit
CTCGTGTGCTTACGAGTTACCATACTTTTCAACCTGTCTCTTATACACATC10.5847953216374269No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA10.5847953216374269No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTA10.5847953216374269No Hit
CTCCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATACCC10.5847953216374269No Hit
CTTGAAAACCATCTTAAAGCAACTCAAAGCTCAAATCGAAATTTCTAGTTT10.5847953216374269No Hit
GTTGACAATCTATGCTCCTTTATTCGCTTCTTCAAAGAGAGCTGTTGTGAC10.5847953216374269No Hit
GTATTTTTAGTAGAGACGGGGTTTCACCTTGTTAGCCCTGTCTCTTATACA10.5847953216374269No Hit
CATATGACCGGTCCTGCTCCATACCTCCATCATTATACTCATTGCTGTCTC10.5847953216374269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTCTTGT10.5847953216374269No Hit
TATCAACGCAGAGTACGGGATGGGGGGACCATCCTCCAAGGCTAAATACTC10.5847953216374269No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAT10.5847953216374269No Hit
GAATAACATTTGCAGAATGTAATATTGTAGCTGTCTCTTATACACATCTCC10.5847953216374269No Hit
CATGTCATTTGTTCATGAGACGAGTCCATATAAGAGCTGTCTCTTATACAC10.5847953216374269No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTAAACCCCCTTGTCTCT10.5847953216374269No Hit
CTCCAGTCACAACCTTTATCGGATCTCGTATGCCGTCTTCTGCTTGAAACA10.5847953216374269Illumina PCR Primer Index 9 (96% over 29bp)
GAGCTTTGTTGACTTTGCTCATGAAAAGCCTCTTCAATATCACACCATTGA10.5847953216374269No Hit
GTACGGGAAAGAGTGTTTTGTTATTAAGATCTCGGTCTGTCTCTTATACCC10.5847953216374269No Hit
GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCCCCGTACT10.5847953216374269No Hit
GTATCAACGCTGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC10.5847953216374269No Hit
GCGTTGTGCATGCGCTCAAATGGCTGCAGACGGCGGCCTGTCTCTTATACA10.5847953216374269No Hit
AAAAAGTACTCTGCGTTGATACCAGTGCTTCTGTCTCTTATACACATCTCC10.5847953216374269No Hit
CCCAGCCACAGGGGCAGCAACACCACCAGCGCAATCGCAAAGCGCAGCCAG10.5847953216374269No Hit
GGATTCCTAAATCCAGTACGAGGGGTCTTTCTTCCCTTCCACTTTTCAGTC10.5847953216374269No Hit
CGCTTGTTGTATTCACAAATCGTATAAGGCGTAAAATGATTTTTGAAGTGT10.5847953216374269No Hit
ACGGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCC10.5847953216374269No Hit
GTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAA10.5847953216374269No Hit
GACAAAAGAAAAGGTCTTTTCAAACAATATGGCTAGTCAAGTTCTGGAATC10.5847953216374269No Hit
ATGATAAACTTAACATGGTCATCAAGGAGTTGAAGGGAAAGAACCTGTCCC10.5847953216374269No Hit
TCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCTGGCC10.5847953216374269No Hit
CTCATACAATACTTGGACAACCATTGGCTTTCATATTACCTGTCTCTTATA10.5847953216374269No Hit
GTACGGGGGGCCCAGACAGCCAGGATGTTGGCTTAGAAGCAGCCATCATTT10.5847953216374269No Hit
ATTCAGTCCAGCTCAGGAAAATTGGGAACATATATTGCTGTCTCTTATACA10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAACATATCTCGTATGCCGTC10.5847953216374269No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCCTGTCTCTTATACACATCTC10.5847953216374269No Hit
TATGAAGATGTTGAAAATTACCTTGAGCCTTCTCCCATATATGCTGTCCCT10.5847953216374269No Hit
ATGCAGAAATTATGCCACGTGGTATGATGAAAAAACGAATGACATGTAGTA10.5847953216374269No Hit
GTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCG10.5847953216374269No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATACACCAAA10.5847953216374269RNA PCR Primer, Index 27 (96% over 28bp)
CTCCAAAACCAATGGAGTCCAATATACGATGTCGCTGCTATACTAACCCTG10.5847953216374269No Hit
ATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGT10.5847953216374269No Hit
GGATACAACTTTCTGTGATGTGAGATCACACTTGTTCCCCTGTCTCTTATA10.5847953216374269No Hit
ATCTTCCTTACTGCTGCCTCCCGTAGGAGTCGGGCCCGTTTCTCAGTGCAC10.5847953216374269No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGATGCCAGTGC10.5847953216374269Illumina PCR Primer Index 8 (96% over 27bp)
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCCTGTCTCT10.5847953216374269No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.5847953216374269No Hit
ATACTGAAGTTAGCAAACTTCTCGTATGCTGGAAACCATGTGTAGAATCCA10.5847953216374269No Hit
AGGGTATCCTGATGGCTGAGGGTATTGTTGTACTTGTGGGTATAGAGATGG10.5847953216374269No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAGAGACGTGT10.5847953216374269No Hit
CGAGTACTTGGAAGAAGACTTACCCAAAAAGAGCACTTTACTTCCTGTCTC10.5847953216374269No Hit
GGTATCAACGCAGAGTACGGGAACGCAGAGTACTTTTTCTGTTCCTTCTCC10.5847953216374269No Hit
GAATATTTGCTACTACCACCAAGATCTGCACCTGTCTCTTATACACATCTC10.5847953216374269No Hit
GAAGGGAGACAGTTCTGCTGAAGAACTGAAACTGGCCACCCAGCTGACCGG10.5847953216374269No Hit
AGGGAATAGTATTGACACCTTAGTATGAAACACCCAACATAACAGAATACG10.5847953216374269No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA10.5847953216374269No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCCTGTCTCTTATCACATCTCCG10.5847953216374269No Hit
GTTCCAAGTAATCTTTCAAACACGTGAACGATCTCTTTCCTGTCTCTTATA10.5847953216374269No Hit
TCCAATCACTGTCTTAAACTAGAAAAAATAAGAGTAACGTAGTCCCAGAAA10.5847953216374269No Hit
GTATCAACGCAGAGTACGGGAGTCTGTCTCTTATACACATCTCCGAGCCCA10.5847953216374269No Hit
GTATCAACGCAGAGTACGGGAAATGCCTTTTAAACTCATAGGCCCACATAG10.5847953216374269No Hit
GGATGAGACCCCGGTCAGCTTCCTTGTCACTGTCTCTTATACACATCTCCG10.5847953216374269No Hit
ATAATACGCAACTCCCAAGAACGCAATCCCGTACCCGAAACGATTAATAGG10.5847953216374269No Hit
ATACAACCTTTTCTGAACTTAAACCTTGCCATCTCCCAGTTCTTTAACTTT10.5847953216374269No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTCTGTCTCTT10.5847953216374269No Hit
AACGAAAAGTGAAGAAAAATGGGAGTAGTACTGATCCTGTCTCTTATACAC10.5847953216374269No Hit
GATTGGACGTAACTTACCTTTGAACATTCCATTAAGCATACCTCCGATGGA10.5847953216374269No Hit
CCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCTGTCTCT10.5847953216374269No Hit
GCCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC10.5847953216374269RNA PCR Primer, Index 20 (95% over 21bp)
CCTTAGATCAGAAGCTTCTCAATAGCATCCTTGAGATGTTGAATCTCTGTC10.5847953216374269No Hit
CTTAATAGTTGATACAAGACAAACATAAACACCTGTCCCTTATACACATCT10.5847953216374269No Hit
TTATAATGCTGATATTTTCATTTCCAGTTTTTATTTTGCTTGCACTCTGTC10.5847953216374269No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCC10.5847953216374269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTTCTTATCTCGTATGCCGTC10.5847953216374269No Hit
TTTTTTATCCTCGTGTGCTTACGAGTTACCATGCTTTTCAACTTCTGTCTC10.5847953216374269No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAAAAA10.5847953216374269Illumina PCR Primer Index 5 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTTCATATCTCGTATGCCGTC10.5847953216374269No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers