FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_35.35100000042389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_35.35100000042389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178207
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC90785.094075990280966No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT20961.1761603079564775No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG3330.18686134663621518No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3000.1683435555281218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.16160981330699692No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2440.1369194251628724No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2350.13186911849702873No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.12794110220137256No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.12176850516534142No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2090.11727934368459152No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1930.10830102072309168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10100.043.2143245
GTATCAC250.00209161636.0375251
CAGAGGT250.002103181635.99707811
ATGCCGT13350.032.69397744
CGCCTCA352.8122222E-432.1402527
GCCTCAT352.8122222E-432.1402528
GGTTCGC352.8122222E-432.1402523
CGAGACC13750.032.07012623
AGACCTC13700.032.0229525
CCACGAG13800.031.95393220
CTCTACT13650.031.8106129
ATCTCGT13750.031.74288237
TATCTCG13750.031.74288236
GACCTCT13750.031.74288226
CACGAGA13900.031.72404921
GAGACCT13850.031.67613224
CCCACGA14000.031.65814819
ACCTCTA13800.031.6278727
TCTCGTA13800.031.6278738
ACGAGAC13950.031.6103422
Produced by FastQC (version 0.11.2)