FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_34.3510000004236f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_34.3510000004236f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324407
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC299529.232846393573505No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT80222.472819637060853No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC29780.9179826575875367No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC15330.4725545379723619No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA14790.4559087812531789No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG14310.44111255305834957No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG14120.4352557127312296No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT13130.4047384920793941No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC12620.3890174996223879No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC11360.3501774006109609No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA9120.2811283357017573No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC8810.2715724383259301No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC8790.27095592881781216No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA7730.23828092488756406No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC7700.23735616062538722No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA7380.22749200849550102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA6550.20190686390860862No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT6030.18587761669754352No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT5790.17847950260012885No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG5380.16584105768371213No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG5340.16460803866747634No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG5310.1636832744052995No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC5180.15967596260253325No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5090.15690166981600273No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC4790.1476540271942344No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC4390.13532383703187661No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4380.13501558227781768No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA4280.13193303473722823No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA4000.12330190162357778No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG3960.12206888260734201No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG3860.11898633506675256No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC3790.11682855178833997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.11405425900180945No Hit
AAAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3590.11066345670716106No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT3580.11035520195310211No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC3510.10819741867468952No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC3450.10634789015033584No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC3360.10357359736380535No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG3280.10110755933133378No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3260.1004910498232159No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGG207.017613E-445.010641
ACTAGTC253.882709E-545.003726
AGGTCAA207.028282E-444.9967617
GATCGTA207.028282E-444.9967615
TAGGTGC207.028282E-444.9967613
ACACACG207.028282E-444.9967612
CACTCAG207.028282E-444.996767
GTCGTCA253.886246E-544.9967634
GAGCATC207.028282E-444.9967637
TGTCGTC253.886246E-544.9967633
TGCCGTC30050.044.02345745
GTCTAGA301.1365335E-437.5088651
CTAGTCT301.1375656E-437.50308227
ATATAGT301.1375656E-437.5030826
CCTGGCA301.1375656E-437.50308226
CACTCCT301.1385986E-437.49730340
TCGTCAT301.1385986E-437.49730335
TGAACGT250.00210403536.0029623
GTCAGTC250.00210403536.0029618
AGCTCTA250.00210403536.002966
Produced by FastQC (version 0.11.2)