FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_32.35100000042339.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_32.35100000042339.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95942
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC1769318.44134998228096RNA PCR Primer, Index 13 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG1044110.88261658085093No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT82078.55412645139772No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGC4670.4867524129161368No Hit
CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTC2450.2553626149131767No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTC1310.13654082674949447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC18800.044.6404445
TATACTG352.8014588E-432.1424835
ATGCCGT26450.031.72931144
CTATACT300.0051243329.9996534
TTACTCA300.0051243329.9996534
AAAAACG300.0051243329.99965316
TACGGGA406.125106E-428.12467415
TATGCCG37150.022.59058643
GTATGCC37250.022.52994242
GAGTACG602.4449007E-422.4997412
AGTACGG602.4449007E-422.4997413
CGTATGC37600.022.3202241
TCTCGTA37850.022.23223738
TCGTATG37800.022.20212240
CTCGTAT37700.022.20133239
ATCTCGT37950.022.05507937
AATCTCG38200.021.85184136
CTATTCA39400.021.41472629
CAAATCT39100.021.406434
TCAAATC39150.021.37906333
Produced by FastQC (version 0.11.2)