FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_31.3510000004231f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_31.3510000004231f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494275
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC12599025.48985888422437RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT227714.606949572606343No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG129382.6175711901269536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC130500.044.4798645
ATGCCGT152750.038.00079344
AACCGCG301.1394177E-437.4975557
GTACTAG250.002098267236.026811
GCCGTCT1400.035.3548445
TATGCCG165850.035.0127943
CTCGTAT166600.034.9767139
ATCTCGT166450.034.9676837
GTATGCC166350.034.94812842
TCTCGTA166850.034.93778638
CGTATGC167250.034.89458541
TCGTATG167650.034.7979140
AATCTCG167150.034.7539436
AAATCTC166850.034.7490135
AGACCTA168750.034.73106825
CCTATTC168450.034.7261428
GACCTAT168650.034.72498326
ACCTATT168600.034.7085927
CTATTCA168450.034.6994329
CGAGACC169050.034.68274323
Produced by FastQC (version 0.11.2)