FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_30.351000000422f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_30.351000000422f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715396
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC194342.7165374142433003RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT82451.152508540724298No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG15150.2117708234320572No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA8750.12230988152016505No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT207.0326816E-444.99846312
TGCCGTC19900.043.64172445
ATGCCGT28500.030.55158644
GCACGGT300.005146834529.99897629
CCCGCAT300.005146834529.99897643
ACCGCGG554.163154E-628.6353868
TATGCCG30800.028.19708843
TATACCG406.1575236E-428.1299385
CGAGACC31200.027.83558723
GTATGCC31350.027.77417242
GACCTAT31050.027.75267626
GAGACCT31350.027.63063424
AGACCTA31250.027.57505625
CGTATGC31600.027.4832441
CCACGAG31850.027.47943720
CACGAGA31900.027.43636721
ATCTCGT31550.027.31285737
TCTCGTA31650.027.29764638
GCCCACG32000.027.28031718
ACGAGAC32200.027.2506222
Produced by FastQC (version 0.11.2)