FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_29.351000000422cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_29.351000000422cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543511
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC7446413.700550678827108RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT191923.531115285615195No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG61441.130427903023122No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA7180.13210404205250675No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC7070.13008016397092237No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA6670.12272060731061561No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6470.11904082898046221No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5790.10652958265794069No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC5750.10579362699191001No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCCG207.031133E-444.99852810
TGCCGTC77300.044.3581945
ACGACCC356.2396703E-638.573725
TGCGGTC250.002106471535.99882545
ATGCCGT98000.035.0116144
TATGCCG104600.032.80246443
CGTATGC105750.032.65850441
CTCGTAT105900.032.59100339
ATCTCGT106200.032.5836837
GTATGCC105400.032.57483742
TCTCGTA106200.032.56249238
TCGTATG106200.032.52012340
AATCTCG106400.032.45899236
AAATCTC106350.032.4107935
GACCTAT106700.032.32555826
CAAATCT106550.032.30771634
AGACCTA106900.032.30717525
CGAGACC107150.032.29776423
ACGAGAC107400.032.26448422
GAGACCT107200.032.24071524