Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_1228_pb_29.351000000422cd.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 543511 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC | 74464 | 13.700550678827108 | RNA PCR Primer, Index 13 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT | 19192 | 3.531115285615195 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG | 6144 | 1.130427903023122 | No Hit |
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA | 718 | 0.13210404205250675 | No Hit |
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC | 707 | 0.13008016397092237 | No Hit |
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA | 667 | 0.12272060731061561 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 647 | 0.11904082898046221 | No Hit |
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC | 579 | 0.10652958265794069 | No Hit |
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC | 575 | 0.10579362699191001 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGATCCG | 20 | 7.031133E-4 | 44.998528 | 10 |
TGCCGTC | 7730 | 0.0 | 44.35819 | 45 |
ACGACCC | 35 | 6.2396703E-6 | 38.57372 | 5 |
TGCGGTC | 25 | 0.0021064715 | 35.998825 | 45 |
ATGCCGT | 9800 | 0.0 | 35.01161 | 44 |
TATGCCG | 10460 | 0.0 | 32.802464 | 43 |
CGTATGC | 10575 | 0.0 | 32.658504 | 41 |
CTCGTAT | 10590 | 0.0 | 32.591003 | 39 |
ATCTCGT | 10620 | 0.0 | 32.58368 | 37 |
GTATGCC | 10540 | 0.0 | 32.574837 | 42 |
TCTCGTA | 10620 | 0.0 | 32.562492 | 38 |
TCGTATG | 10620 | 0.0 | 32.520123 | 40 |
AATCTCG | 10640 | 0.0 | 32.458992 | 36 |
AAATCTC | 10635 | 0.0 | 32.41079 | 35 |
GACCTAT | 10670 | 0.0 | 32.325558 | 26 |
CAAATCT | 10655 | 0.0 | 32.307716 | 34 |
AGACCTA | 10690 | 0.0 | 32.307175 | 25 |
CGAGACC | 10715 | 0.0 | 32.297764 | 23 |
ACGAGAC | 10740 | 0.0 | 32.264484 | 22 |
GAGACCT | 10720 | 0.0 | 32.240715 | 24 |