FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_28.351000000422a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_28.351000000422a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270945
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC3756413.864068353355846RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT56112.070899998154607No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG53321.9679270700695712No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG3130.11552160032478917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC40050.044.04277845
ATGCCGT46250.038.13866444
ACCGTAC250.002104781335.9982268
CTCGTAT52100.034.02903439
TATGCCG51900.033.98676743
TCGTATG52250.033.93134740
TCTCGTA52450.033.84485638
GTATGCC52200.033.7914442
CGTATGC52500.033.76976841
ATCTCGT52650.033.7162937
AATCTCG52650.033.7162936
AAATCTC52650.033.5880935
TCAAATC52850.033.46098333
ATTCAAA52900.033.38682631
TTCAAAT53050.033.37724732
CAAATCT52950.033.35529734
ACCTATT53200.033.24084527
CTATTCA53000.033.2389329
TATTCAA53050.033.20760330
CCTATTC53250.033.1673828
Produced by FastQC (version 0.11.2)