H23LNBCXX_l02n01_bir_1228_pb_27.35100000042297.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pb_27.35100000042297.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475374
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC	9058	1.905447079562618	RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT	2106	0.44301960140857516	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	789	0.16597458001489354	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA	582	0.12242991833798231	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	580	0.12200919696912327	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	558	0.11738126191167375	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG	523	0.11001863795664045	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	515	0.10833575248120426	No Hit

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1005	0.0	43.65335	45
GCATGAG	35	6.243863E-6	38.56846	7
GTACCGT	25	0.0021066659	35.997223	6
GCGGTCT	25	0.0021066659	35.997223	11
ATGCCGT	1240	0.0	35.380333	44
TATGCCG	1295	0.0	33.877697	43
ACGAGAC	1295	0.0	33.707508	22
CGTATGC	1295	0.0	33.703964	41
CGAGACC	1290	0.0	33.663734	23
CTCGTAT	1315	0.0	33.533535	39
CACGAGA	1305	0.0	33.44921	21
GTATGCC	1315	0.0	33.362446	42
GACCTAT	1295	0.0	33.36001	26
ATCTCGT	1310	0.0	33.32155	37
GAGACCT	1310	0.0	33.32155	24
CCACGAG	1310	0.0	33.32155	20
TCTCGTA	1335	0.0	33.034634	38
AATCTCG	1315	0.0	32.85263	36
CCCACGA	1330	0.0	32.82047	19
AGACCTA	1330	0.0	32.82047	25