FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_24.3510000004223a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_24.3510000004223a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75222
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC35224.682140863045386No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT12611.676371274361224No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG4750.6314641993033953No Hit
TTGTTTCTCAGTAGTATCAAGCATACATTTTCCTTTCTGTCTCTTATACAC1120.148892611204169No Hit
ATCCTTCAAAGAAAATGAATGATGTTGCATTACGTAAGGCAATTGCTTATG920.12230464491771025No Hit
TCTTTATCATCAGTTTTGCTTCTGTGAGATTCAATAGTAACTGTCTCTTAT920.12230464491771025No Hit
GTAATAATGCTTTGTACGGTATAGATACTGATCACTAAGACTGTCTCTTAT870.11565765334609557No Hit
ATATAGCACAAGGAGTTTTAAGTTATGGATCTTGAGATAAGGAGACTGTCT840.11166945840312675No Hit
TGTCAAAAAAGTTCAAAGTGATGCAACTTACAAAGAGGGTTACAACAAAGG830.11034006008880382No Hit
GAAGTATAGTCACAATGTCACTTTAGTTTCAGGTGTACAGCATAGTAGATA810.10768126346015792No Hit
AAAAGGAATTTTTCACTTCATTCTTCAATTGCATGTTGTTAGTGCAAATAC810.10768126346015792No Hit
AGATAGTAACATCTGTTGGTTGAGTTGTTATTGAAGGAAGTGTATTAACCT810.10768126346015792No Hit
ACATAGTAATCATCCACCTTAAATGTGATGTTCCCGTTTTTATCTTTTACC760.10103427188854325No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT253.856017E-545.024
CTCAACT206.9912645E-445.04
AGGACAG206.9912645E-445.034
AAGTTGC206.9912645E-445.039
CAGTGAA206.9912645E-445.017
TCTCAAC253.856017E-545.03
TGGGATA206.9912645E-445.033
ACTCACA253.856017E-545.023
TGCCGTC3450.041.08695645
GTACCAC301.1298078E-437.50000410
CATTAGA301.1298078E-437.50000428
GTTACCA301.1298078E-437.50000435
GAGTTAC301.1298078E-437.50000433
GACAGGT250.002094637136.036
GTTCTGC250.002094637136.08
TTAGACA250.002094637136.04
ATTGTTA250.002094637136.018
CTGGTAA250.002094637136.020
AGTTGCA250.002094637136.040
GGCATAT250.002094637136.023
Produced by FastQC (version 0.11.2)