FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_23.35100000042210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_23.35100000042210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151575
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC2364715.600857661223817No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT90755.9871350816427515No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG73644.858320963219528No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2460.1622958931222167No Hit
TGTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCC1930.12732970476661717No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1760.1161141349167079No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC1730.1141349167079004No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT1590.10489856506679862No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTTA206.995406E-445.0240976
GGTCGTC207.018204E-444.9943928
TGCCGTC23600.043.27850345
CTTGAAA601.5279511E-1037.49532727
AGGGTGA250.00209243936.0311785
CAGGGTG250.00209243936.0311784
CAACGAA250.002102639835.99551427
ACACCTC250.002102639835.9955147
CACCTCG250.002102639835.9955148
GAGTTAC250.002102639835.99551426
TGTGACA458.642255E-734.9956367
GTGACAA458.642255E-734.9956368
TCTAAAA352.7946694E-432.1706922
GAAATCG352.8109673E-432.1388543
ATGATAC352.8109673E-432.1388536
ATGTCGC352.8109673E-432.138857
CCGTCTT708.021743E-1032.1388517
GATGATA352.8109673E-432.1388535
TGTCGCT352.8109673E-432.138858
CCCCCGG855.456968E-1231.76074618
Produced by FastQC (version 0.11.2)