FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_22.351000000421f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_22.351000000421f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160876
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC30031.8666550635271888No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT26741.6621497302270072No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG5420.33690544270121087No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2520.1566423829533305No Hit
ATAAGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTA2130.13240010940102936No Hit
ATACGAGATAAGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAG1680.10442825530222034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGG453.8016879E-1044.9959633
CCGGGGG453.8016879E-1044.9959635
GAACCGG453.8016879E-1044.9959632
TGCCGTC2950.043.47067345
GGGGAAC501.0695658E-940.49636538
ACCGGGG501.0695658E-940.49636534
CGGGGGA552.7212081E-936.81487336
TACCGTG250.002096251836.019165
ACCGTGA250.002099455836.007966
CGAACCG504.83833E-835.9967731
CGTGAGG250.002102663535.996778
GGGGGAA606.364644E-933.7469737
TAAGACT501.9594827E-631.5167664
GTCGTAA300.00510534330.0346531
CCGTGAG300.00513651429.9973077
AGGCGAA602.3990287E-729.99730728
TAGGTTA803.3523975E-928.12247518
GAACTGA654.8334186E-727.68982141
ATGAGGC654.8334186E-727.68982125
GGGAACT654.8334186E-727.68982139