FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_21.351000000421db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_21.351000000421db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274121
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC38651.409961294464853No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT22670.8270070516304844No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG5150.18787323846038792No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTC207.0252793E-444.9981778
AACCTCC207.0252793E-444.99817729
TGCCGTC4150.042.8295945
AGGACTA301.13792674E-437.4984837
GGAGGAC250.002102851236.0051086
ACTATGG250.002104735235.9985412
GGACTCA250.002104735235.998549
GTGCTCA250.002104735235.998549
GGTCTAG250.002104735235.9985415
ACAGTCG606.3973857E-933.7486348
TAGACAG754.7293724E-1133.004685
GACAGTC651.3958925E-831.1525827
CTAAACG300.005136935530.0042573
GAGGACT300.005141521329.9987857
GTCTAGT300.005141521329.99878516
CGATGTG300.005141521329.9987859
CGTTTGC300.005141521329.99878515
CTACACC702.865454E-828.9326784
GGTTGTC702.8705472E-828.9273997
CTATATG554.1451603E-628.6404293
Produced by FastQC (version 0.11.2)