FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_20.351000000421b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_20.351000000421b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162918
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC7580.4652647344062657No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT3840.23570139579420324No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG2080.12767158938852674No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTA207.0159225E-444.999399
TGCCGTC1250.039.59946445
TTTGAGC250.002101956635.9995083
GGAGCGG300.005134792529.999598
AGGACCG300.005134792529.999595
TATTATA300.005134792529.999592
CTTAGGA300.005134792529.999592
TATGCCG1800.028.74960743
ATGCCGT1750.028.28532844
ATCTCGT1800.027.49962437
CCCACGA1850.026.7563919
GTCTTAT1850.026.7563932
GTATGCC1950.026.538142
CTCGTAT1900.026.05227739
CAGTGTC1900.026.05227728
GACAGTG1950.025.3842726
CGTATGC1950.025.3842741
TATCTCG1950.025.3842736
CACGAGA2050.025.24355921
GTATCAA5400.025.0150171
Produced by FastQC (version 0.11.2)