FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_16.3510000004212e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_16.3510000004212e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87049
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC65847.56355615802594No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT12051.384277820537858No Hit
TACCAATATTGTTATTGATTTCATCGAACAAAGACCACAGGATTTATTTTC4870.5594550195866695No Hit
AATAGAATCGAAACGAAATGAATCGAAGAGAATCGATTAGAATCGAAATGA3430.3940309480867098No Hit
ATTTGTGTGTTACATTTTAACACTTGACTCAGATGAATTATGGCCTTTTAC2940.33774081264575123No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG2690.3090213557881193No Hit
TAATAGCAGTGTACCAAATAAGCAGTAATGAACAAACTGTAAAACTAACTG2420.2780043423818769No Hit
ATACTATTCTGGAACATTCTTGTTCAACTTTTTCATGTGGTTATTGGATTT2350.26996289446173993No Hit
CTTTTACAGTATGCTTGTCGTAAACTAAAATAGTTGCATAAGCATTCATTA2350.26996289446173993No Hit
GAATTAGCTGTTACTATTGACAAAGAAGGAAATGTAACTATCGTTACAAAA2290.26307022481590825No Hit
GTTATACTAACAGCTACAACTACTGGTAATGGAACATGTAGTCCTATTGTT2200.2527312203471608No Hit
TGTCTTCACACTTTATTTCGTTAAGTTGATCTTCAATTTCTGATATCCTTT2200.2527312203471608No Hit
GGAATATTAAATAGTTCCTGAATGAGTGCTTATGATTTCTCAGTATAGAAC2130.2446897724270239No Hit
GCTAAAAAGGATTACCAAATTCTTATCCATGTTCCTAAATCGTTAACAACA2080.2389458810554975No Hit
GGAAAATGCTATTGATAAAATGTCGTATCAAGAAATCATCGAAATGATTCA2060.23664832450688691No Hit
AATCATAGCTAAACAATAAGATTTCAGTTCAGGATCTTTAATGGCATAGTA2010.23090443313536052No Hit
TAGTAAGATCGGATTCAATTTCTTTTTCTTTTCCGCCCAGATATTTGAACA1910.2194166503923078No Hit
GACTTAGTACAAAGTAGAAAGTGACAGAACTGAAATGAAAATATGGTGGAT1870.21482153729508666No Hit
AACATCAATATCGATGATAGGAGGTACATCTTCTTTGGCATATTTTAACGC1860.21367275902078142No Hit
GCTCTAATCTTTATTATTTCCCTTCTTCTGCTGACTTTAGGCTTTATCTGC1830.21022642419786558No Hit
GTGTTAAGAAAACTGATAGTAAAATACGACATTTCAATCTACAATGAAGAG1810.20792886764925503No Hit
AAACCATAACTAATACGACACCAATAATTGCGTACATCGCTAATATGTCTC1730.1987386414548128No Hit
GAAGAAAAGAGTATTTTTTCTAATAGCTCGTTTCTACGTATTTCAAGTTCT1720.19758986318050756No Hit
ATATAACACCGAGCAATTAATTTGAGAAATGAACTTTGGCAGTTATTTAGA1710.19644108490620227No Hit
ATGATCAGGATTCATTTGTGATCATGAATGGATTATTGTGTCATATATTAA1710.19644108490620227No Hit
AATCACTAAGTGATAATTCATTTATCGAAATCACCTTTAGCTCTTCTTTTT1700.19529230663189698No Hit
GTTGATTTATAGGGTGATAACTTCAAAGAAGGATTTTAGGAATAGTGGAAC1690.1941435283575917No Hit
ACTGACTATTGACTACTGACTATTGACTAATGACTATTGACTAATGACCAA1660.19069719353467587No Hit
GAATTAGTAGGACAAATTGTTGAAGGTCCATTTAATGCAATACCACCTTCT1570.18035818906592838No Hit
TTATTCAAGTACCAATATGCAATACTGATGAGACAAAAAGAAGTGAAGAAT1560.1792094107916231No Hit
AAATTATCTTTGGTGATTACGTTTTTGAATGTGTTATCTTCCAGTAATTCA1550.17806063251731785No Hit
ATATTACTCAGTGCATTTTAATGAGAGTGACATGTGTATCCCTGAGAAATG1540.17691185424301256No Hit
ATATGTTAGATCCTAAATCTAGTCACAGAAGAAGGAAAAGAAAATTGAACC1530.17576307596870727No Hit
TTTCAAGATTGTTTCAGAAATTCTGGTTATATTGCATGTCCACATTAATCT1510.17346551942009672No Hit
ATTCTTTTCGATTCTTTTTGATTCGGTTTGATTCTTTTCGATTCAATTCGA1500.17231674114579146No Hit
GATTAGTGATGTTGAACATTTTTTTCATATGTTTTTTGGCCATTTGCGTAT1490.17116796287148617No Hit
ATGTACAATTATTTGCTTGATAACGGAATGACACGAAAAGAATACGAGTGG1470.16887040632287562No Hit
ATAGTGTAATACTGTCATAAAGGCAAACATATAGACCAATGGAATAGAATC1450.16657284977426506No Hit
GCAATTGTATAGTCAAGGTTTCTATTTCTAAGTGGCTGATTAAACCATAAA1450.16657284977426506No Hit
AGGTATACCTGTCAAGAATTATGATAAAGGATTACCAAACAGAGAATCGGT1410.16197773667704396No Hit
ACATTACTTGGAATAGTCCAAGGAGTAGTAGTCCAAATTACTAGTGATGTA1390.1596801801284334No Hit
TAAAAACACACTTAACTAATGAAGTCATCAGGAAAATGCAAATTAAAACCA1390.1596801801284334No Hit
AAATTAAATATCTCAACGAATGTGCTGATTAGGGAGTATTAGTTATTAGTT1380.15853140185412815No Hit
GTAATATCTGTCCTTTTTAGATCATTTTCGTCTAATCGTGAAAATATTCTA1380.15853140185412815No Hit
TTACTGTATAGATCCATTGTTGTTTCTTATCTTGCTTTCTTTGATTGTACT1360.15623384530551757No Hit
AATAAGTACAGCTATTTTTCCATTGGTTAAATTTCCAATCATAATATCCCA1360.15623384530551757No Hit
GATTACAAAATCGTAGAAAGAAACTAGATCCATATGAAGCTGTTATTGTTG1340.15393628875690704No Hit
TCTTTCTCTTTGTAAGGCAATTTGATAGCTGGATATGTATAAATACCTGAA1330.15278751048260175No Hit
AATCAGTAGTAAGGTTTATAAAAGCAAAAGTAACAATAATTCCATGCGAGG1310.1504899539339912No Hit
GATTTGTTTCGACTCATTTCTTTTCTATTCGATTCTTTTGATTCGATTCTT1310.1504899539339912No Hit
AATTAAGCCTTTTATTTATTACTGGCATCATGTAGATGGTAATCTAACCTT1300.1493411756596859No Hit
CAATAACCTAATAACTAAATTGGACTACACATTCGAGAAACAAAAAAATGT1280.14704361911107536No Hit
AAATAATGATTTGCCATACTATTTATCAGATCAATGCTTTAATTTGTTCCC1260.14474606256246483No Hit
GAGTTTATTTCTCAAAGTTCTAGTTGTGAACAGTAAATGAGAGGTTTTTAT1240.14244850601385425No Hit
CTTTATATGTAACCATATTTGACAAACAGAACAATGAAAAAGCAACCATTT1220.1401509494652437No Hit
AGTTACGACAGCATACAATCAAATGTTGATACACTTATAAAACGCTTGCAA1190.1367046146423279No Hit
TTTTGAATCATTGTGTTCATCAATATTTCTACAGTGGTTGAGAAAAGTCAA1170.13440705809371734No Hit
ATACATTACTGAGTGATCTCTTTCTATAATCTCTTTGCAGTATTATTCCAC1170.13440705809371734No Hit
CCCAAAATATAGTCTTTTAGAACCTGTGGAGATAAAAAGGAATAAGTCAAA1160.13325827981941205No Hit
GATATGATCCGTATCGTTGAAATGATTGATAGCGTATTAATGAATCAAACT1160.13325827981941205No Hit
TATTAAGGAAGATAACATGGCATTTTTAATTGCCAACAAAATCAAATCAAT1140.1309607232708015No Hit
GTATTTAACAGCTTTGTTAGTATTACTCTTTAACGATGCGAGATGATAATA1140.1309607232708015No Hit
GCTTATGATAATGGTTTAGAAAATGGCAATTGGATATACAAAAACAACGGT1140.1309607232708015No Hit
TTTGAAATTAGTATCTGATGGTGCATAATTTTTGCTTTTTAAACCTATATA1140.1309607232708015No Hit
GTATATTACTACTTTGTCAAAAAACACTCTGAATTCTTCTAAGAATGCTTT1130.12981194499649623No Hit
ATGATAAAGGATCCATTATTTAGGTTGTTAGAGGGAGAGAACTTGAAACAG1120.12866316672219097No Hit
GAATATTGCCTTTACATCAATTTGAAAACAATGTTTCCTTTGATGTTTGCT1120.12866316672219097No Hit
ACAGGATGTAATCTTAACATAGGATTAGACTCTTCCGAAATTAGGATCGAT1120.12866316672219097No Hit
ATACTAATCAAACTAGCATAATACTGGTTGTCTATTGCTTACTATTATGCG1110.12751438844788568No Hit
AATTATTTGAGACCATTCAGGAAAACTTTGATGCAAATTATATTGGCGAAA1100.1263656101735804No Hit
AATATGAAGTCCTTGAATATGGATATGGTATGTGTTTTTCAAGCGTATAAT1090.12521683189927513No Hit
GAATTGATTCGATTAGATTCTTTTCGATTTCATTTGATTAGTTTCGATTCT1090.12521683189927513No Hit
TATGTAAATAGATCGCATCTGTAATAGATACCATCGCAACCTTAAATGTAT1090.12521683189927513No Hit
ATATTACGCAAGAAGAAAAAAGTGTTATCTATACAGCAACTGTCAAAAACC1080.12406805362496984No Hit
TTTTAATGTTATTCCATTATGTGCTGATTTCCATAACTTCTGTTGAAAAGT1080.12406805362496984No Hit
AAGAAAATGACACTCTAAAATACAACTAGGTCAAACTTTCTAGAAGATAAA1060.1217704970763593No Hit
TTATGACAGAGGTATACTCAGACAAGTTTATCAAAACACTTCTATGGAACT1040.11947294052774875No Hit
GATGTAAACACAAAACAATACATGCTTTCGGGTTTATATAATTTCAGTTGG1020.11717538397913818No Hit
ATCTCAGACTCATTCATTGTTTAGTGTTAATTTGGAAGAAATATCAGATGC1010.1160266057048329No Hit
ATTTTCTCTATTCTTCTAGTTGGATAATTTTTCTTTCCCTTTTCTAGGAGA970.1114314926076118No Hit
GTAATAATCCAACACTTTTATATGGTTATGGAGGATTTAGCTCAAGTCTTA960.11028271433330653No Hit
TAGAAGAACTGTCTGGTAAATATGTTGATCGTAAAAGCCAATTAAAGAAGA950.10913393605900125No Hit
ATATCATGTATTTGCATAGTTGGGTTTTCAGTAATTGTGATAAAAAGCAAA940.10798515778469597No Hit
ACTTTTTTGTTGTTGTTGCTTATGCTTTTTATGTCATATCCAAGAAAGTAT930.10683637951039071No Hit
AATTGAAAATAATCGAATCGAATCGAAAATAATCAAATCGAATCGAAAATA930.10683637951039071No Hit
TTACATGATGAGTAGTTTTACTGAATGTTCTTAACTTTTTTCTAAACTTGC920.10568760123608543No Hit
TCTAAATACAGCTCTCATAAAATGCATAAGAGATCCTTGGTAAGTATCGTA910.10453882296178015No Hit
CAAATAGATGAACTTTAAGTTGATTCAATTCTACATGGACAACCATAAATA910.10453882296178015No Hit
TGGAATGGAATTGAAAGGAATCATCAAATGGACTTGAATGAAATCATTGAA910.10453882296178015No Hit
CTAAATAACAGGTTAATAACAAACATTTTCATTCAAGAAACTGGGTTCTTA900.10339004468747487No Hit
TAGTTCTTCACATACAAGTATCAGGACATACTGATTAAATAACCCCTGAGT900.10339004468747487No Hit
AAATTGGATGTTTGTTATCAAAATAACCTTGGTGAACTATTCCATCTACAA900.10339004468747487No Hit
TCTAATAACTGAGTAAGTTTTTCAATCATTCGTTCTCTTAGAACTAAAAGA890.1022412664131696No Hit
GGAAGAAAATAAGTTTATTCGTTTTCATTGGTTACATTCCGATAAAAATGA880.10109248813886432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTAGT351.194694E-745.00000436
CAGATGA351.194694E-745.00000430
CATAGCT351.194694E-745.0000044
TGATCAG253.861052E-545.02
TATCAGC253.861052E-545.045
GACAGAA253.861052E-545.023
GGTACAT253.861052E-545.023
ATCTAGT406.690243E-945.017
CAAGTCT206.9973525E-445.043
ATAGGGT406.690243E-945.09
AAGAGGT206.9973525E-445.07
CCTAGAA206.9973525E-445.035
CCCTAGA206.9973525E-445.034
GTCCTTA206.9973525E-445.018
CTTCAAA453.765308E-1045.021
GTCCTAT253.861052E-545.041
GAGTATG206.9973525E-445.01
TCTAGTC406.690243E-945.018
ACTTACG206.9973525E-445.04
GTAATCC206.9973525E-445.027
Produced by FastQC (version 0.11.2)